PMID: 8606179Apr 1, 1996Paper

Amino acids induce expression of BAP2, a branched-chain amino acid permease gene in Saccharomyces cerevisiae

Journal of Bacteriology
T DidionH A Andersen

Abstract

Branched-chain amino acid uptake in Saccharomyces cerevisiae is mediated by at least three transport systems: the general amino acid permease Gap1p, the branched-chain amino acid permease Bap2p, and one or more so far unknown permeases. Regulation of the transcription of BAP2 is mainly subject to the presence of certain amino acids in the medium. The level of transcription is low during growth on a minimal medium with proline as the sole nitrogen source. As assayed with a lacZ fusion, the level of transcription is slightly higher (3-fold) on a minimal medium with ammonium ions as a nitrogen source, and transcription is induced about 20-fold by addition of leucine (0.2 mM). As little as 10 microM leucine causes a fivefold induction. Addition of (L)-leucine to minimal proline medium, on the other hand, has no effect on BAP2 transcription. The two known permeases for transport of branched-chain amino acids, Gap1p and Bap2p, are thus not active at the same time. The BAP2 promoter contains one or two putative Gcn4p binding sites and one putative Leu3p binding site. None of the three is needed for induction by leucine. Induction of BAP2 transcription by leucine is accompanied by an increase in branched-chain amino acid uptake. This e...Continue Reading

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Citations

Jan 26, 2005·Biochemical Society Transactions·P PoulsenM C Kielland-Brandt
Jan 16, 2009·Biochemical Society Transactions·Per O Ljungdahl
May 17, 2013·Applied Microbiology and Biotechnology·Kristoffer Krogerus, Brian R Gibson
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May 23, 1997·The Journal of Biological Chemistry·M B WrightR F Gaber
Nov 20, 2016·Journal of Experimental Botany·Julien BesnardSakiko Okumoto
Sep 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Mario D GarciaLuke W Guddat
May 8, 2003·Yeast·Per NørgaardJakob R Winther
Jan 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Harinder S Hundal, Peter M Taylor
Apr 5, 2014·Journal of Amino Acids·Daniel A SáenzCarlos A Stella
Mar 1, 2008·Molecular Membrane Biology·Peter PoulsenMorten C Kielland-Brandt
Feb 29, 2008·Annual Review of Genetics·Shadia ZamanJames R Broach
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
Aug 11, 2016·Applied Microbiology and Biotechnology·Martina AvbeljPeter Raspor
May 3, 2006·The Journal of Cell Biology·Boqian WuMorten C Kielland-Brandt
Nov 19, 2008·Applied Microbiology and Biotechnology·Bart A SmitGerrit Smit
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Sep 15, 2015·Journal of Agricultural and Food Chemistry·Martina AvbeljPeter Raspor
Jun 20, 2002·Genetics·Laura K PalmerRalph L Keil

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