Amino-terminal fragment (1-34) of parathyroid hormone-related protein inhibits migration and proliferation of cultured vascular smooth muscle cells

Atherosclerosis
M IshikawaY Ouchi

Abstract

We investigated the effects of amino-terminal fragment (1-34) of parathyroid hormone-related protein [PTHrP-(1-34)] on the migration and proliferation of vascular smooth muscle cells (VSMCs). Cultured VSMCs (5-9th passage) obtained from the aortas of male Wistar rats were used in this study. Migration of VSMCs was assessed using a modified Boyden's chamber. Proliferation of VSMCs was evaluated by measuring [3H]thymidine incorporation and counting cell numbers. PTHrP-(1-34) inhibited 10% fetal bovine serum (FBS)-induced increase in migration of VSMCs (61% of control at 1 micromol/l) in a concentration-dependent manner. PTHrP-(1-34) also inhibited 5% FBS-induced increase in [3H]thymidine incorporation (37% of control at 1 micromol/l) and cell number of VSMCs (33% of control at 1 micromol/l) in a concentration-dependent manner. Parathyroid hormone (PTH)-(1-34) inhibited the migration and DNA synthesis of VSMCs to a similar extent. PTHrP-(7-34), a PTH/PTHrP receptor antagonist, significantly inhibited these effects of PTHrP and PTH. PTHrP-(1-34) also inhibited platelet-derived growth factor-BB (5 ng/ml)-induced migration and DNA synthesis of VSMCs. These findings suggest that PTHrP-(1-34) inhibits the migration and proliferation of...Continue Reading

References

Feb 15, 1989·Biochemical and Biophysical Research Communications·K NagasakiK Abe
Nov 30, 1987·Biochemical and Biophysical Research Communications·R J WinquistG P Vlasuk
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M ManginD E Barton
Sep 1, 1988·The Journal of Clinical Investigation·J N WilcoxD Gordon
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J M MoseleyJ D Zajac
Feb 20, 1986·The New England Journal of Medicine·R Ross
Nov 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T TsukadaA M Gown
Jan 1, 1985·Circulation Research·A W Clowes, S M Schwartz
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R GrotendorstG R Martin
Jan 28, 1994·Biochemical and Biophysical Research Communications·E RianK M Gautvik
Mar 15, 1994·Biochemical and Biophysical Research Communications·M IshikawaH Orimo

❮ Previous
Next ❯

Citations

Jul 28, 2001·Kidney International·M R Davies, K A Hruska
Jul 29, 2008·International Journal of Clinical Practice·H S ChoiS-K Lim
Sep 17, 2010·Journal of Molecular and Cellular Cardiology·Gyun Jee SongAlessandro Bisello
Jun 14, 2003·Stroke; a Journal of Cerebral Circulation·José Luis Martín-VenturaJesús Egido
Sep 19, 2014·Journal of the American Society of Nephrology : JASN·Gérard M LondonMarie-Christine de Vernejoul
Mar 22, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T MassfelderJ J Helwig

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.