Amino-terminal precursor sequence modulates canine distemper virus fusion protein function

Journal of Virology
Veronika von Messling, Roberto Cattaneo

Abstract

The fusion (F) proteins of most paramyxoviruses are classical type I glycoproteins with a short hydrophobic leader sequence closely following the translation initiation codon. The predicted reading frame of the canine distemper virus (CDV) F protein is more complex, with a short hydrophobic sequence beginning 115 codons downstream of the first AUG. To verify if the sequence between the first AUG and the hydrophobic region is translated, we produced a specific antiserum that indeed detected a short-lived F protein precursor that we named PreF(0). A peptide resulting from PreF(0) cleavage was identified and named Pre, and its half-life was measured to be about 30 min. PreF(0) cleavage was completed before proteolytic activation of F(0) into its F(1) and F(2) subunits by furin. To test the hypothesis that the Pre peptide may influence protein activity, we compared the function of F proteins synthesized with that peptide to that of F proteins synthesized with a shorter amino-terminal signal sequence. F proteins synthesized with the Pre peptide were more stable and less active. Thus, the Pre peptide modulates the function of the CDV F protein. Interestingly, a distinct two-hit activation process has been recently described for human...Continue Reading

References

Oct 1, 1991·Computer Applications in the Biosciences : CABIOS·I LadungaT Geszti
Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
Nov 1, 1990·Nature·B MossT R Fuerst
Nov 1, 1993·AIDS Research and Human Retroviruses·M A MillerR C Montelaro
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y LiC Y Kang
Feb 1, 1997·Journal of Virological Methods·H SchneiderM A Billeter
Jan 10, 1998·The EMBO Journal·B MartoglioB Dobberstein
Oct 28, 1998·Trends in Cell Biology·B Martoglio, B Dobberstein
Jun 20, 2001·Journal of Virology·V von MesslingR Cattaneo
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·L González-ReyesJ A Melero
Sep 6, 2001·The Journal of Biological Chemistry·R K PlemperR Cattaneo

❮ Previous
Next ❯

Citations

Jan 13, 2005·Acta Neuropathologica·Marc Vandevelde, Andreas Zurbriggen
May 26, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tanner S MiestRoberto Cattaneo
Apr 23, 2008·The Journal of Biological Chemistry·Jin K LeeRichard K Plemper
Jun 19, 2009·Briefings in Bioinformatics·Jan A Hiss, Gisbert Schneider
Sep 13, 2003·Journal of Virology·Veronika von Messling, Roberto Cattaneo
Jun 15, 2004·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·María J Forzán, Salvatore Frasca
Nov 28, 2015·Journal of Virological Methods·Carina RomanuttiJosé La Torre
Nov 18, 2008·The Veterinary Journal·Ming-Shiuh LeeWei-Li Hsu
Feb 6, 2020·Frontiers in Microbiology·Jumpei AsoHajime Takizawa
Mar 9, 2019·Virology Journal·Santiago Rendon-MarinJulian Ruiz-Saenz

❮ Previous
Next ❯

Related Concepts