Amino-terminal processing of cell surface heparin-binding epidermal growth factor-like growth factor up-regulates its juxtacrine but not its paracrine growth factor activity.

The Journal of Biological Chemistry
T NakagawaN Taniguchi

Abstract

Human heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) expressed on Chinese hamster ovary (CHO) cells is synthesized as a 19-kDa major, and 22- and 27-kDa minor, membrane-anchored precursors (proHB-EGF). In contrast, the 27-kDa species is major and the 19- and 22-kDa ones are minor in mouse proHB-EGF. The juxtacrine growth factor activities of human and mouse proHB-EGFs on CHO cells toward EP170.7 cells in co-culture are significantly different. To investigate the relationship between the juxtacrine growth factor activities and the molecular species, we prepared human-mouse chimeras. Chimeras that have the human amino-terminal sequence with a mouse EGF-like domain showed approximately 8-fold up-regulation of the juxtacrine growth factor activity and the predominance of a 19-22-kDa major species. In contrast, chimeras that have the mouse amino-terminal sequence with a human EGF-like domain showed approximately 5-fold down-regulation of the juxtacrine activity and the predominance of the 27-kDa major species. A Gly32.HB-EGF (117-amino acid form), which is amino-terminally extended, induced the same mitogenic activity as that of Arg73.HB-EGF (75-amino acid form), which is amino-terminally truncated. These ...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C I BrannanN G Copeland
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P AnklesariaJ Massagué
Aug 1, 1985·Cell·P StroobantM D Waterfield
Mar 31, 1995·The Journal of Biological Chemistry·H ToyodaK Hanada
May 19, 1995·The Journal of Biological Chemistry·N ToyodaP R Larsen
Jan 14, 1994·Biochemical and Biophysical Research Communications·N ItoY Matsuzawa
Jan 1, 1993·Annual Review of Biochemistry·J Massagué, A Pandiella
Mar 12, 1993·Science·Y ShingJ Folkman

❮ Previous
Next ❯

Citations

Feb 18, 1999·Journal of Cellular Biochemistry·H TadaH Yamada
Jan 13, 1998·Biochimica Et Biophysica Acta·G Raab, M Klagsbrun
Feb 7, 2009·Tissue Engineering. Part a·Jean-Jacques GicquelAndrew Hopkinson
Jan 24, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Peter J BuontempoBahige M Baroudy
Oct 31, 2002·The Journal of Cell Biology·Ketu Mishra-GorurSpyros Artavanis-Tsakonas
Mar 12, 2015·Molecular Medicine Reports·Gong-Ping Wang, Xiao-Fang Han
Aug 30, 2008·Biochemical and Biophysical Research Communications·J T HoskinsP A Harding
Feb 12, 2005·American Journal of Respiratory Cell and Molecular Biology·Eric K ChuDaniel J Tschumperlin
Aug 30, 2003·The Journal of Biological Chemistry·Sylvain M Le GallPhilippe Mauduit
Aug 25, 2020·Current Opinion in Otolaryngology & Head and Neck Surgery·Peter Luke Santa Maria
Jun 29, 2004·Biochemical and Biophysical Research Communications·Daisuke NanbaShigeki Higashiyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.