PMID: 6990419Apr 1, 1980Paper

Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form

Proceedings of the National Academy of Sciences of the United States of America
D L OxenderC Yanofsky

Abstract

A 2.1-kilobase Bgl II DNA fragment from Escherichia coli containing livK, the gene coding for the leucine-specific binding protein, has been cloned into the BamHI site of the plasmid vector pBR322. The DNA sequence of segments of the resulting plasmid, pOX7, established the location of the livK gene and the direction of its transcription. In vitro protein synthesis directed by pOX7DNA yielded the Mr 42,000 precursor of the leucine-specific binding protein and a small amount of the Mr 39,000 mature protein. Continued incubation of the in vitro reaction mixture after DNase and RNase treatment resulted in additional processing. The DNA sequence of the beginning of livK suggested that 23 additional amino acid residues are present as an extension of the NH2 terminus of the mature protein. Amino acid sequence analysis established that the precursor has the predicted 23-residue extension. Proteolytic digestion studies with the precursor and mature forms of the leucine-specific binding protein indicate that there are conformational differences between the two. This suggests a possible role for the signal sequence in determining the conformation of the binding protein precursor that is recognized by the membrane.

References

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Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D L OxenderC Yanofsky

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Citations

Aug 1, 1991·Journal of Cellular Biochemistry·M D Adams, D L Oxender
Jan 1, 1983·The International Journal of Biochemistry·I Suominen, P Mäntsälä
Jan 1, 1985·The International Journal of Biochemistry·B R Moore, S J Free
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T TaniguchiM Ptashne
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C J DanielsD L Oxender
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Müller, G Blobel
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A MinskyJ R Knowles
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R FittsD Botstein
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R M Williamson, D L Oxender
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik
Jul 11, 1984·Nucleic Acids Research·M E Watson
May 15, 1981·European Journal of Biochemistry·G von Heijne
May 1, 1990·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·R H WainbergM B Yatvin
Sep 23, 1985·FEBS Letters·D M Burns, I R Beacham
Jan 1, 1980·Journal of Supramolecular Structure·C J DanielsD L Oxender
Jan 1, 1980·Journal of Supramolecular Structure·R LandickD L Oxender
Apr 1, 1994·Journal of Bacteriology·M M Mackle, B A Zilinskas
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Jan 1, 1984·Journal of Cellular Biochemistry·B R CopelandD L Oxender

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