Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients

The Journal of General Virology
Xiaofen ZhongZhihong Guo

Abstract

The 3a protein of severe acute respiratory syndrome (SARS)-associated coronavirus is expressed and transported to the plasma membrane in tissue cells of infected patients. Its short N-terminal ectodomain was found to elicit strong humoral responses in half of the patients who had recovered from SARS. The ectodomain-specific antibodies from the convalescent-phase plasma readily recognized and induced destruction of 3a-expressing cells in the presence of the human complement system, demonstrating their potential ability to provide immune protection by recognizing and eliminating SARS coronavirus-infected cells that express the target protein. In addition, when coupled to a carrier protein, the ectodomain peptide elicited 3a-specific antibodies in mice and rabbit at high titres. These results showed that the N terminus of the 3a protein is highly immunogenic and elicits potentially protective humoral responses in infected patients. Therefore, the short extracellular domain may be a valuable immunogen in the development of a vaccine for infectious SARS.

References

Apr 3, 2003·The New England Journal of Medicine·Susan M PoutanenUNKNOWN Canadian Severe Acute Respiratory Syndrome Study Team
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
Jan 21, 2004·Acta Biochimica Et Biophysica Sinica·Ping ZhaoZhong-Tian Qi
Jan 31, 2004·Biochemical and Biophysical Research Communications·Mau-Sun ChangYuh-Cheng Yang
Jan 31, 2004·Science·UNKNOWN Chinese SARS Molecular Epidemiology Consortium
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shiou-Hwei YehUNKNOWN National Taiwan University SARS Research Team
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himani BishtBernard Moss
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ursula J BuchholzPeter L Collins
Jun 25, 2004·The Journal of Infectious Diseases·Danny Tze Ming LeungPak Leong Lim
Aug 4, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Yulin ShiCheng Cao
Aug 20, 2004·The New England Journal of Medicine·Robert E Johnston
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeShibo Jiang
Jan 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhi-yong YangGary J Nabel

❮ Previous
Next ❯

Citations

Nov 30, 2007·Virus Research·Krishna NarayananShinji Makino
Apr 10, 2007·Virus Research·Luis EnjuanesRalph Baric
Apr 29, 2009·The International Journal of Biochemistry & Cell Biology·Chak-Ming ChanHo Yin Edwin Chan
Jul 6, 2014·Antiviral Research·Ding Xiang LiuRolf Hilgenfeld
Jun 6, 2012·International Journal of Cell Biology·Fulvio ReggioriAnne Simonsen
Oct 27, 2020·Epidemiology and Infection·Parinita Majumdar, Sougata Niyogi
Nov 7, 2020·Frontiers in Immunology·Conor McClenaghanColin G Nichols
Jun 24, 2021·Nature Structural & Molecular Biology·David M KernStephen G Brohawn
Sep 19, 2021·Nature Reviews. Microbiology·Haitao Yang, Zihe Rao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.