Aminoacetone, a putative endogenous source of methylglyoxal, causes oxidative stress and death to insulin-producing RINm5f cells

Chemical Research in Toxicology
Adriano SartoriEtelvino J H Bechara

Abstract

Aminoacetone (AA), triose phosphates, and acetone are putative endogenous sources of potentially cytotoxic and genotoxic methylglyoxal (MG), which has been reported to be augmented in the plasma of diabetic patients. In these patients, accumulation of MG derived from aminoacetone, a threonine and glycine catabolite, is inferred from the observed concomitant endothelial overexpression of circulating semicarbazide-sensitive amine oxidases. These copper-dependent enzymes catalyze the oxidation of primary amines, such as AA and methylamine, by molecular oxygen, to the corresponding aldehydes, NH4(+) ion and H2O2. We recently reported that AA aerobic oxidation to MG also takes place immediately upon addition of catalytic amounts of copper and iron ions. Taking into account that (i) MG and H2O2 are reportedly cytotoxic to insulin-producing cell lineages such as RINm5f and that (ii) the metal-catalyzed oxidation of AA is propagated by O2(*-) radical anion, we decided to investigate the possible pro-oxidant action of AA on these cells taken here as a reliable model system for pancreatic beta-cells. Indeed, we show that AA (0.10-5.0 mM) administration to RINm5f cultures induces cell death. Ferrous (50-300 microM) and Fe(3+) ion (100 mic...Continue Reading

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Citations

Sep 18, 2012·Endocrine·Paulo MatafomeRaquel Seiça
Nov 8, 2014·IUBMB Life·Sangeeta ChakrabortyDipshikha Chakravortty
Aug 8, 2015·Chemical Research in Toxicology·Sireesh DornadulaRamkumar Kunka Mohanram
Mar 23, 2011·Mutation Research·Georgia-Persephoni VoulgaridouAglaia Pappa
Sep 17, 2016·Medicinal Research Reviews·Paulo MatafomeRaquel Seiça
Dec 8, 2019·Nature Communications·Alba LuengoMatthew G Vander Heiden
Feb 27, 2021·Free Radical Biology & Medicine·Luiz D RamosEtelvino J H Bechara

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