PMID: 1544Dec 29, 1975

Aminoacyl transfer from an adenylate anhydride to polyribonucleotides

Journal of Molecular Evolution
A L Weber, J C Lacey

Abstract

Imidazole catalysis of phenylalanyl transfer from phenylalanine adenylate anhydride to the hydroxyl groups of homopolyribonucleotides was investigated as a chemical model of the biochemical aminoacylation of tRNA. Imidazole catalyzed transfer of phenylalanine to poly(U) increases from pH 6.5 to 7.7 and decreases above pH 7.7. At pH 7.7 approximately 10% of the phenylalanyl residues are transferred to poly(U). At pH 7.1, transfer to poly(U) was five times as great as to poly(A) and transfer to a poly(A) poly(U) double helix was negligible. At pH 7.1 approximately 45 mole percent linkages to poly(U) were monomeric phenylalanine; the remainder of the linkages were peptides of phenylalanine. The number of linkages and their lability to base and neutral hydroxylamine indicates that phenylalanine and its peptides are attached as esters to the 2' hydroxyl groups throughout poly(U) and the 2' (3') hydroxyl groups at the terminus of poly(U). These results do model the contemporary process of aminoacyl transfer to tRNA and continue to suggest that a histidine residue is in the active site of aminoacyl-tRNA-synthetases.

References

Aug 17, 1973·Nature·R Lohrmann, L E Orgel
Jan 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T Nakashima, S W Fox
Mar 17, 1973·Biochemical and Biophysical Research Communications·W E WhiteA L Weber
Aug 24, 1973·Biochimica Et Biophysica Acta·A L Weber, S W Fox
May 17, 1974·Biochimica Et Biophysica Acta·A L Weber, J C Lacey
May 12, 1972·Biochemical and Biophysical Research Communications·J C Lacey, W E White
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G Kampitz, S W Fox
Feb 1, 1970·Die Naturwissenschaften·T NakashimaS W Fox
Apr 3, 1968·European Journal of Biochemistry·M BELJANSKIP Bourgarel
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Citations

Jan 1, 1984·Origins of Life·D A UsherK M Lo
Aug 2, 1978·Journal of Molecular Evolution·A L Weber, J C Lacey
May 12, 1978·Journal of Molecular Evolution·A L Weber, L E Orgel
Jan 1, 1981·Journal of Molecular Evolution·J R Hawker, J Oró
Dec 1, 1980·Journal of Molecular Evolution·D H White, J C Erickson
Jan 1, 1985·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·J C LaceyD W Mullins
Jan 1, 1992·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·N S Wickramasinghe, J C Lacey
Jan 1, 1985·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·J C LaceyC L Watkins
Jan 11, 1992·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·J C LaceyG W Cook
Jan 1, 1987·Journal of Molecular Evolution·A L Weber
Jan 1, 1984·Journal of Molecular Evolution·M KinjoK Nagano
Dec 1, 1989·Journal of Molecular Evolution·L E Orgel
Feb 3, 1995·Science·M IllangasekareM Yarus
Dec 26, 2009·Journal of Theoretical Biology·H James Cleaves
Mar 19, 1991·Biochemistry·N S WickramasingheJ C Lacey
Jun 9, 2006·The FEBS Journal·Malgorzata Giel-Pietraszuk, Jan Barciszewski
Feb 2, 2021·Biochemistry·Aleksandar RadakovicJack W Szostak

Related Concepts

5'-Adenylic Acid
Amino Acyl-tRNA Synthetases
Catalysis
Biological Evolution
Hydrogen-Ion Concentration
Imidazoles
Polypeptides
Endorphenyl
Adenine Polynucleotides
Poly U

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