PMID: 6990972May 13, 1980Paper

Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu

Biochemistry
A Pingoud, C Urbanke

Abstract

Hydrolysis protection experiments were used for a quantitative determination of the binding of several aminoacyl-tRNAs to the Escherichia coli elongation factor Tu. The observed differences could not be rationalized in terms of structural properties of the tRNAs. The experimental results support, however, a model according to which the differences in the affinity of naturally occurring aminoacyl-tRNAs are determined mainly by the nature of the amino acid esterified to the tRNA. Aminoacyl-tRNAs with polar amino acid side chains are bound less strongly than those with apolar ones. This model is substantiated by results obtained with misacylated and modified aminoacyl-tRNAs. Furthermore, it could be shown that the aminoacyl group of the aminoacyl-tRNA must be in the L configuration; EF-Tu in this way prevents blocking of the ribosomal A site or even incorporation of D-amino acids into protein. The data have been used for a schematic description of the structure of a part of the aminoacyl-tRNA binding site of the bacterial elongation factor Tu.

References

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Citations

Mar 27, 1981·Molecular and Cellular Biochemistry·A Parmeggiani, G Sander
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Sep 2, 2003·Current Opinion in Structural Biology·Adam FrankelRichard W Roberts
Jul 17, 2008·Biochemistry·Terry J T CathopoulisTamara L Hendrickson
Feb 12, 2004·Nucleic Acids Research·Anne Théobald-DietrichJoëlle Rudinger-Thirion
Jun 1, 1981·European Journal of Biochemistry·A Pingoud, W Block
Jun 15, 1982·European Journal of Biochemistry·W FischerM Sprinzl
Mar 10, 1990·European Journal of Biochemistry·S TapioM Ehrenberg
Dec 5, 2013·ELife·Sadeem AhmadRajan Sankaranarayanan
Dec 27, 2005·Journal of Molecular Biology·Katrin Karbstein, Jennifer A Doudna
Nov 2, 2005·Trends in Biochemical Sciences·Taraka Dale, Olke C Uhlenbeck
May 31, 2015·Nucleic Acids Research·John AchenbachSven Klussmann
Nov 26, 2005·Molecular Cell·Katrin KarbsteinJennifer A Doudna
Jun 13, 1998·The Journal of Biological Chemistry·T D CaldasG Richarme

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