Aminoacyl-tRNA enrichment after a flood of labeled phenylalanine: insulin effect on muscle protein synthesis

American Journal of Physiology. Endocrinology and Metabolism
G CasoM A McNurlan

Abstract

Muscle protein synthesis in dogs measured by flooding with L-[(2)H(5)]phenylalanine (70 mg/kg) was significantly stimulated by infusion of insulin with amino acids. The stimulation of muscle protein synthesis was similar when calculated from the enrichment of phenylalanyl-tRNA (61 +/- 10%, P < 0.001), plasma phenylalanine (61 +/- 10%, P < 0.001), or tissue fluid phenylalanine (54 +/- 10%, P < 0.001). The time course for changes in enrichment of L-[(2)H(5)]phenylalanine throughout the flooding period was determined for plasma, tissue fluid, and phenylalanyl-tRNA in the basal state and during the infusion of insulin with amino acids. Enrichments of plasma free phenylalanine and phenylalanyl-tRNA were equalized between 20 and 45 min, although the enrichment of phenylalanyl-tRNA was lower at early time points. Rates of muscle protein synthesis obtained with the flooding method and calculated from plasma phenylalanine enrichment were comparable to those calculated from phenylalanyl-tRNA and also to those obtained previously with a continuous infusion of phenylalanine with phenylalanyl-tRNA as precursor. This study confirms that, with a bolus injection of labeled phenylalanine, the enrichment of aminoacyl-tRNA, the true precursor poo...Continue Reading

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Citations

Sep 4, 2008·American Journal of Physiology. Endocrinology and Metabolism·Abdul JaleelK Sreekumaran Nair
Apr 16, 2009·American Journal of Physiology. Endocrinology and Metabolism·Heath G GasierJames D Fluckey
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Feb 25, 2005·American Journal of Physiology. Endocrinology and Metabolism·James GibneyKen K Y Ho
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Sep 2, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Thomas C VaryAndrew Sumner
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Wen LongZhenqi Liu
May 30, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
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Oct 24, 2007·Clinical Science·Giuseppe CasoMargaret A McNurlan
Feb 8, 2007·The American Journal of Clinical Nutrition·Giuseppe CasoMargaret A McNurlan
May 1, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
Jun 18, 2011·Expert Review of Proteomics·Mary K Doherty, Phillip D Whitfield

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