PMID: 36832Jun 1, 1979

Aminoglycoside-3'-phosphotransferase from Actinomyces fradiae. Its isolation, purification and properties

Antibiotiki
V L GanelinS M Navashin

Abstract

Aminoglycoside phosphotransferase was isolated from the mycelium of Act. fradiae, the neomycin-producing organism, with paromomycin, neomycin and to a less extent ribostamycin being substrates of aminoglycoside-phosphotransferase. It was purified to homogenous state. The maximum activity of the enzyme preparations was observed at pH 7.7--7.8;KM for neomycin and paromomycin was about 20 micron and KM for ATP was 150 micron. Mg2+ ions were necessary for the enzyme activity. None of the divalent cations tested could replace the magnesium ions in the reaction of phosphorylation catalyzed by the enzyme. High sensitivity to the ionic strength of the buffer was characteristic of the enzyme. It lost about 80 per cent of the initial activity at a concentration of KC1 equal to 1.0 M. The molecular mass of the enzyme from the mycelium of Act. fradiae was determined by the method of gel-filtration through sefadex G-100. It was about 22,000. High stability was characteristic of the enzyme. The fingings indicate that aminoglycoside phosphotransferase from Act. fradiae differs from the described aminoglycoside-3'-phosphotransferases isolated from antibiotic resistant bacteria.

Related Concepts

Cations, Divalent
Aminoglycoside [EPC]
Filtration
Alkalescens-Dispar Group
Aminoglycosides
Actinomyces
Protein Phosphorylation
MUC7 gene
Enzyme agent
Enzyme Activity

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