Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.

Plant Cell Reports
I M G Padilla, L Burgos

Abstract

Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material. The bacterial gene nptII, coding for a neomycin phosphotransferase, and the hpt gene, coding for a hygromycin phosphotransferase, are frequently used. These enzymes detoxify aminoglycoside antibiotics by phosphorylation, thereby permitting cell growth in the presence of antibiotics. Nevertheless, the screening for transgenic regenerated shoots is often partial and difficult due to regeneration of escapes and chimeras. These difficulties can be caused, in part, by an incorrect assumption about the mode of action of antibiotics in bacterial and eukaryotic cells and in in vitro tissue culture. The information contained in this review could be useful to establish better selection strategies by taking into account factors such as explant complexity, transformation and selection protocols that allow better accessibility to cells of Agrobacterium and antibiotics, and faster regeneration methods that avoid collateral effects of antibiotics on recovered, putative transgenic shoots.

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Citations

May 27, 2014·Journal of Chemical Ecology·Jyothilakshmi VadasseryAxel Mithöfer
Dec 14, 2011·International Journal for Parasitology·Gabriel RinaldiPaul J Brindley
Feb 7, 2013·The British Journal of Dermatology·J P McFaddenI Kimber
Jun 3, 2015·Journal of Natural Products·Camilo F Martinez-FarinaDavid L Jakeman
Mar 12, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Amanpreet KaurAnil Kumar
Oct 30, 2021·Frontiers in Genome Editing·Jannis RinneMarco Herde

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