PMID: 9432027Jan 31, 1998Paper

Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA-dependent pseudothrombocytopenia

British Journal of Haematology
S SakuraiK Nakahara

Abstract

Although EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is of practical importance because failure to recognize this clinical entity may result in misdiagnosis and subsequent mismanagement of the patients, the pathophysiological nature of EDTA-PTCP remains unknown. To develop an effective way to evaluate the platelet counts in patients with EDTA-PTCP, we introduced aminoglycosides-supplemented anticoagulating agents. When kanamycin was pre-supplemented with EDTA for anticoagulating blood samples from EDTA-PTCP patients there was no significant change in the platelet counts and the morphology of blood cells after 150 min of incubation at room temperature. Furthermore, when kanamycin was added to EDTA-anticoagulated blood samples from EDTA-PTCP patients within 30 min after blood withdrawal, rapid dissociation of platelets without apparent morphological changes of blood cells was observed, and complete blood cell counts as well as the histogram patterns were almost the same as those examined immediately after blood sampling. The dissociation of aggregated platelets was also detected when other antibiotics were used, although it was associated with some extent of morphological changes of blood cells. These findings indicate that...Continue Reading

Citations

Jul 25, 2000·Journal of Clinical Laboratory Analysis·Y KinoshitaN Tatsumi
Nov 15, 2002·International Journal of Hematology·Yeon S Ahn, Lawrence L Horstman
Jan 16, 2014·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Preethi PaulNavneet Kumar
Jul 18, 2000·Journal of the American College of Cardiology·D C SaneR M Califf
Jun 6, 2009·Veterinary Clinical Pathology·Deanna M W SchaeferNicole Belcher
Mar 22, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·A J LombartsP F Franck
May 9, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe BanfiGiuseppe Lippi
Jun 22, 2014·BMJ Case Reports·Cem SahinTugba Dibektas Canbek
Jan 10, 2012·The American Journal of the Medical Sciences·Fatih OzcelikIsmail Kurt
May 25, 2013·The American Journal of Emergency Medicine·Naoko OhashiNaoki Yahagi
Jan 17, 2007·International Journal of Laboratory Hematology·M ZandeckiA Godon
May 4, 2011·British Journal of Haematology·Kate HodgsonEmili Montserrat
Nov 27, 2014·Journal of Clinical Laboratory Analysis·Jianhua LinMiaohuan Kuang
May 17, 2002·The Korean Journal of Internal Medicine·Hae Lyun AhnChun Jo Jin
Oct 17, 2012·Platelets·Arjan AlbersenPaul Hamberg
Nov 6, 2001·The Tohoku Journal of Experimental Medicine·R TsujiT Takubo
Sep 13, 2001·Clinical and Laboratory Haematology·J J Hoffmann
Sep 16, 2011·American Journal of Clinical Pathology·Xiaomian ZhouWenshen Luo
Jun 10, 2010·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Sinan AkbayramAhmet Faik Oner
Mar 20, 2020·Journal of Clinical Medicine·Véronique BacciniValérie Bardet
Feb 10, 2021·Journal of Clinical Medicine·Benjamin LardinoisFrançois Mullier
Mar 23, 2021·Case Reports in Medicine·Enise CeranBarbara Steinlechner
Apr 6, 2002·Clinics in Laboratory Medicine·Bakul I Dalal, Malcolm L Brigden
Jul 31, 2021·International Journal of Laboratory Hematology·Ting LiYun Zhang

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