Aminopyrine N-demethylase activity in human liver microsomes

Clinical Pharmacology and Therapeutics
J A García-AgúndezJ Benítez

Abstract

Aminopyrine N-demethylase activity and the contents of cytochrome P-450, cytochrome b5, and NADPH-reductase activity in human liver microsomes from 31 different patients were studied. Our results show the existence of significant interindividual, but not sex- or age-related differences in N-demethylase activity (ranging between 0.52 and 4.42 nmol/min/mg protein), and that these differences are directly correlated with the content of cytochrome P-450 and NADPH-reductase activity, but not with that of cytochrome b5 content, in the samples. In contrast, no differences were found in the Michaelis-Menten constant values for aminopyrine (mean, 2.4 mmol/L) or NADPH (mean, 69 mumol/L), strongly suggesting that interindividual differences could be due to the occurrence of different amounts of the same enzyme, rather than the presence of different enzymes, in the population studied.

Citations

Apr 1, 1995·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J A AgúndezJ Benítez
Feb 1, 1996·Therapeutic Drug Monitoring·J A Agúndez, J Benítez
Sep 25, 2020·Scientific Reports·Javier Gómez-TabalesCarlos Gutierrez-Merino

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