Ammonia-induced extracellular accumulation of taurine in the rat striatum in vivo: role of ionotropic glutamate receptors

Neurochemical Research
M ZielińskaJ Albrecht

Abstract

Accumulation of taurine (Tau), glutamate (Glu) and glutamine (Gln) was measured in vivo in microdialysates of the rat striatum following a direct application to the microdialysis tube of 60 mM ammonium chloride which renders the final ammonia concentration in the extracellular space to approximately 5 mM. The following compounds were coadministered with ammonia to distinguish between the different mechanisms that may underlie the accumulation of amino acids: ion transport inhibitors, diisothiocyanostilbene-2,2'-disulfonate (DIDS) and furosemide, a Glu transport inhibitor L-trans-pyrrolidine-2,4-dicarboxylate (PDC), an NMDA receptor antagonist dizocilpine (MK-801) and an 2-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)/kainate (KA) receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX). Ammonia stimulated Tau accumulation in the microdialysates to approximately 250% of the basal value. Furosemide did not significantly affect the stimulation by ammonia and DIDS only moderately depressed the effect. The ammonia-dependent Tau accumulation was increased by approximately 50% in the presence of PDC and reduced by approximately 35% in the presence dizocilpine and DNQX. In the microdialysates ammonia stimulated Glu and Gln a...Continue Reading

Citations

Dec 20, 2005·Neurochemical Research·Jan Albrecht, Arne Schousboe
Dec 31, 2005·Metabolic Brain Disease·Jan Albrecht, Michał Wegrzynowicz
Apr 24, 2008·Journal of Neuropathology and Experimental Neurology·Arumugam R JayakumarMichael D Norenberg
Jun 26, 2010·Journal of Magnetic Resonance Imaging : JMRI·Aparna SinghalM Albert Thomas
Jun 10, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wojciech HilgierJan Albrecht
Jul 27, 2010·Biochemical Pharmacology·Jan AlbrechtMichael D Norenberg

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