Ammonia manipulates the ammonia-oxidizing archaea and bacteria in the coastal sediment-water microcosms

Applied Microbiology and Biotechnology
Yan ZhangDonghui Wen

Abstract

Ammonia was observed as a potential significant factor to manipulate the abundance and activity of ammonia-oxidizing microorganisms (AOMs) in water environments. For the first time, this study confirmed this phenomenon by laboratory cultivation. In a series of estuarine sediment-coastal water microcosms, we investigated the AOM's phylogenetic composition and activity change in response to ammonia concentration. Increase of ammonia concentration promoted bacterial amoA gene abundance in a linear pattern. The ratio of transcribed ammonia-oxidizing bacteria (AOB) amoA gene/ammonia-oxidizing archaea (AOA) amoA gene increased from 0.1 to 43 as NH4 (+)-N increased from less than 0.1 to 12 mg L(-1), and AOA amoA transcription was undetected under 20 mg NH4 (+)-N L(-1). The incubation of stable isotope probing (SIP) microcosms revealed a faster (13)C-NaHCO3 incorporation rate of AOA amoA gene under 0.1 mg NH4 (+)-N L(-1) and a sole (13)C-NaHCO3 utilization of the AOB amoA gene under 20 mg NH4 (+)-N L(-1). Our results indicate that ammonia concentration manipulates the structure of AOM. AOA prefers to live and perform higher amoA transcription activity than AOB in ammonia-limited water environments, and AOB tends to take the first contr...Continue Reading

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Citations

Jul 6, 2016·Water Research·Nikolaos V ParanychianakisNicolas Kalogerakis
May 11, 2017·Frontiers in Microbiology·Jatta SaarenheimoMarja Tiirola
Dec 17, 2020·Frontiers in Microbiology·João Pereira SantosCatarina Magalhães

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