Ammonia movement in the small intestine: preferential transport by the ileum

The Journal of Clinical Investigation
S M Mossberg, G Ross

Abstract

Isolated, surviving sacs of everted small intestine were used to characterize ammonia transport in the golden hamster. Jejunal and ileal sacs incubated aerobically in ammonia-free test solution liberated the same quantity of ammonia as did sacs that were filled and immediately emptied of their contents, indicating no significant evolution of metabolic ammonia. Under aerobic conditions, ileal sacs transferred a solution of high ammonia content from the mucosal surface to the serosal surface against a concentration gradient. This transport was not glucose dependent and exhibited first-order Michaelis-Menten kinetics. Inhibition of absorption occurred with anaerobiosis, 2,4-dinitrophenol, and sodium cyanide. In jejunal segments ammonia was not transported against an adverse chemical gradient. Ileal ammonia absorption was accompanied by bicarbonate secretion and acidification of the serosal solution. Both bicarbonate movement and pH gradients were abolished by inhibitors of ammonia transport. In the jejunum, the absence of ammonia movement occurred in association with minimal bicarbonate secretion and no appreciable change in serosal pH. Despite the creation of hydrogen ion gradients tending to augment or to retard ammonia absorpti...Continue Reading

References

Feb 1, 1955·The Journal of Clinical Investigation·L P WHITES SHERLOCK
Oct 6, 1956·Lancet·A M DAWSONW H SUMMERSKILL
Mar 1, 1957·The Journal of Clinical Investigation·W H SUMMERSKILLC S DAVIDSON
Apr 1, 1957·Biochimica Et Biophysica Acta·T H WILSON, L KAZYAK
Jul 1, 1957·The American Journal of Medicine·W H SUMMERSKILLC S DAVIDSON
Sep 20, 1957·The Journal of General Physiology·P F CURRAN, A K SOLOMON
May 1, 1958·The American Journal of Medicine·M D MILNEM A CRAWFORD
Dec 1, 1958·The Journal of Clinical Investigation·K S WARREN, D G NATHAN
Jan 20, 1959·The Journal of General Physiology·I L COOPERSTEIN, C A HOGBEN
Feb 1, 1960·The Journal of Clinical Investigation·P A BROMBERGC E FORKNER
Jul 1, 1960·The Journal of General Physiology·P F CURRAN

❮ Previous
Next ❯

Citations

Jun 1, 1994·Journal of Cellular Physiology·J C SeagraveJ R Martinez
Jan 1, 1990·Comparative Biochemistry and Physiology. A, Comparative Physiology·H J Harlow, R A Nelson
Dec 24, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·H J Harlow, E J Braun
Jul 23, 1970·The New England Journal of Medicine·E WolpertW H Summerskill
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K SinghW F Boron
Nov 1, 1972·Biological Reviews of the Cambridge Philosophical Society·M J Carter
Apr 1, 1975·Gut·J H Cummings
Oct 20, 2010·Drug and Chemical Toxicology·Timur V SchäferVladimir L Rejniuk
Jan 1, 2011·ISRN Toxicology·Jury Ju IvnitskyVladimir L Rejniuk
Jun 13, 1998·The American Journal of Gastroenterology·B M DasaniC S Lieber
Sep 6, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Julian G RubinoChris M Wood
Nov 23, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Roger T WorrellJeffrey B Matthews
May 1, 1992·The Journal of Urology·W S McDougal
Apr 16, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Khalid AbdounHolger Martens
Dec 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mary E HandlogtenI David Weiner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.