Ammonia transport in cultured gill epithelium of freshwater rainbow trout: the importance of Rhesus glycoproteins and the presence of an apical Na+/NH4+ exchange complex

The Journal of Experimental Biology
T K N TsuiChris M Wood

Abstract

The mechanisms of ammonia excretion at fish gills have been studied for decades but details remain unclear, with continuing debate on the relative importance of non-ionic NH(3) or ionic NH(4)(+) permeation by various mechanisms. The presence of an apical Na(+)/NH(4)(+) exchanger has also been controversial. The present study utilized an in vitro cultured gill epithelium (double seeded insert, DSI) of freshwater rainbow trout as a model to investigate these issues. The relationship between basolateral ammonia concentration and efflux to apical freshwater was curvilinear, indicative of a saturable carrier-mediated component (K(m)=66 micromol l(-1)) superimposed on a large diffusive linear component. Pre-exposure to elevated ammonia (2000 micromol l(-1)) and cortisol (1000 ng ml(-1)) had synergistic effects on the ammonia permeability of DSI, with significantly increased Na(+) influx and positive correlations between ammonia efflux and Na(+) uptake. This increase in ammonia permeability was bidirectional. It could not be explained by changes in paracellular permeability as measured by [(3)H]PEG-4000 flux. The mRNA expressions of Rhbg, Rhcg2, H(+)-ATPase and Na(+)/H(+) exchanger-2 (NHE-2) were up-regulated in DSI pre-exposed to amm...Continue Reading

Citations

Apr 21, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·C Michele Nawata, Chris M Wood
Jun 22, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Alex M ZimmerChris M Wood
Oct 14, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Carol Bucking, Chris M Wood
Jan 19, 2012·Fish Physiology and Biochemistry·Charles A BrownChristopher C Green
Nov 21, 2009·The Journal of Biological Chemistry·Tsutomu NakadaShigehisa Hirose
Oct 12, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Alex M ZimmerChris M Wood
Feb 8, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher A CooperPatricia A Wright
Mar 15, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shanshan LiShigehisa Hirose
Aug 23, 2012·Respiratory Physiology & Neurobiology·Patricia A Wright, Chris M Wood
Mar 8, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Greg G GossFernando Galvez
Aug 25, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marvin H Braun, Steve F Perry
Aug 25, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·J Moreira-SilvaJ M Wilson
Jul 22, 2009·Respiratory Physiology & Neurobiology·Li Zhang, Chris M Wood
Jun 12, 2013·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Carol BuckingPatrick J Walsh
Jul 30, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hon Jung LiewGudrun De Boeck
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Dec 4, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Li ZhangChris M Wood
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Aug 14, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hon Jung LiewGudrun De Boeck
Dec 30, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Julian G RubinoChris M Wood
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Jan 14, 2020·Respiratory Physiology & Neurobiology·Junho EomChris M Wood
Mar 31, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alex M ZimmerChris N Glover

Related Concepts

Ammonia
Carrier Proteins
Mesothelium
Gill Structure
Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer
Membrane Glycoproteins
Permeability
Sodium
Water-Electrolyte Balance
Receptor Up-Regulation

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