PMID: 2498287Jun 1, 1989Paper

Ammonium inhibition of nitrogenase activity in Herbaspirillum seropedicae

Journal of Bacteriology
H Fu, R H Burris

Abstract

The effect of oxygen, ammonium ion, and amino acids on nitrogenase activity in the root-associated N2-fixing bacterium Herbaspirillum seropedicae was investigated in comparison with Azospirillum spp. and Rhodospirillum rubrum. H. seropedicae is microaerophilic, and its optimal dissolved oxygen level is from 0.04 to 0.2 kPa for dinitrogen fixation but higher when it is supplied with fixed nitrogen. No nitrogenase activity was detected when the dissolved O2 level corresponded to 4.0 kPa. Ammonium, a product of the nitrogenase reaction, reversibly inhibited nitrogenase activity when added to derepressed cell cultures. However, the inhibition of nitrogenase activity was only partial even with concentrations of ammonium chloride as high as 20 mM. Amides such as glutamine and asparagine partially inhibited nitrogenase activity, but glutamate did not. Nitrogenase in crude extracts prepared from ammonium-inhibited cells showed activity as high as in extracts from N2-fixing cells. The pattern of the dinitrogenase and the dinitrogenase reductase revealed by the immunoblotting technique did not change upon ammonium chloride treatment of cells in vivo. No homologous sequences were detected with the draT-draG probe from Azospirillum lipofer...Continue Reading

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Citations

Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
Dec 23, 2011·Molecular Microbiology·Abhijit SarkarBarbara Reinhold-Hurek
Apr 9, 2015·World Journal of Microbiology & Biotechnology·Li-hong Zhang, San-feng Chen
Dec 7, 2005·Archives of Microbiology·Lilian NoindorfLeda S Chubatsu
Jun 4, 2020·World Journal of Microbiology & Biotechnology·Jing-Yi GouChristopher Rensing
Dec 1, 1990·Journal of Bacteriology·A L Lobo, S H Zinder
Mar 1, 1990·Applied and Environmental Microbiology·R S Oremland
Jun 25, 2003·Protein Expression and Purification·Adriana L TwerdochlibLiu U Rigo
Jan 13, 2011·BMC Microbiology·Lilian NoindorfLeda S Chubatsu

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