Abstract
Successful viral infection involves a series of interactions between the virus and the host cell. The outcome of viral infection is, in fact, dependent on intact cellular function; it is required for viral binding, internalization, and uncoating. To determine the potential importance of lysosomal processing on the outcome of infection with a nonenveloped virus, we have studied the effects of NH4Cl on the course of reovirus infection on a beta-cell tumor in culture. Addition of 10 mM NH4C1 to the medium inhibited viral growth by greater than 80% and reduced toxic effects of the virus on cell viability, protein, and DNA synthesis by 30-45%. In addition, synthesis of viral proteins was markedly decreased. Uptake of virus prelabeled with [35S]methionine was not affected by the ammonium; however, cleavage of mu1C, an outer capsid protein of the virus whose cleavage appears to be required for viral replication, was delayed. These results suggest that intracellular processing of reovirus is dependent on a lysosomal pathway and that disruption of this pathway can alter the course of viral infection.
References
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Aug 1, 1978·Cell·F R MaxfieldM C Willingham
Aug 11, 1978·Science·T OnoderaA L Notkins
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H L WeinerB N Fields
Mar 1, 1985·The Journal of General Virology·H ZeichhardtK O Habermehl
Jun 1, 1985·Endocrinology·E Maratos-FlierC R Kahn
Feb 1, 1985·The Journal of General Virology·J BraunwaldF Tripier-Darcy
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·A J Shatkin, J D Sipe
Sep 1, 1972·Virology·W K Joklik
Dec 1, 1971·Virology·C T Chang, H J Zweerink
Aug 15, 1970·Nature·U K Laemmli
Dec 30, 1968·Biochemical and Biophysical Research Communications·J Borsa, A F Graham
Jan 1, 1984·Journal of Cellular Biochemistry·D J Yamashiro, F R Maxfield
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A F GazdarV Lauris
Oct 30, 1982·Virology·A H Sharpe, B N Fields
May 8, 1981·Science·M S BrownJ L Goldstein
Jun 1, 1981·The Journal of Experimental Medicine·T OnoderaA L Notkins
Jan 1, 1982·Annual Review of Biochemistry·G Ashwell, J Harford
Feb 25, 1983·Science·W M Canning, B N Fields
Apr 15, 1983·Science·M V HaspelA L Notkins
Jun 1, 1984·Virology·E C CarrilloR H Campos
May 1, 1980·The Journal of Infectious Diseases·H L WeinerB N Fields
Feb 1, 1980·The Journal of Cell Biology·A HeleniusE Fries
Apr 1, 1981·Diabetologia·J W YoonA B Jenson
Jan 1, 1982·The Journal of General Virology·A HeleniusJ White
Dec 1, 1980·The Journal of Cell Biology·K Sandvig, S Olsnes
Sep 25, 1980·Journal of Molecular Biology·M Marsh, A Helenius
Citations
Jan 1, 1989·Pharmacology & Therapeutics·G D DianaJ Badger
Nov 1, 1988·Annales De L'Institut Pasteur. Immunology·W V WilliamsM I Greene
Jun 8, 2012·Journal of Virology·Bernardo A Mainou, Terence S Dermody
Jan 25, 2008·Journal of Virology·Melissa S MaginnisTerence S Dermody
Jan 21, 2011·Journal of Virology·Bernardo A Mainou, Terence S Dermody
Dec 28, 2005·Journal of Virology·Kimberly M ClarkTerence S Dermody
Jul 4, 2013·MBio·Bernardo A MainouTerence S Dermody
Sep 1, 1991·The Journal of Clinical Investigation·M L NibertB N Fields
Mar 3, 2004·Biotechnology and Bioengineering·Sunghoon JungPatrick J Farrell
Jul 16, 2014·Frontiers in Oncology·Jun Gong, Monica M Mita
Dec 7, 2017·PLoS Pathogens·Angela K BergerBernardo A Mainou
Jun 26, 2015·Journal of Virology·Bernardo A MainouTerence S Dermody
Sep 1, 1993·Journal of Virology·P Lucia-JandrisB N Fields
Jan 1, 1996·Journal of Virology·C G MartínezL Carrasco
Oct 1, 1994·Journal of Virology·H W VirginK L Tyler
Apr 1, 1988·Journal of Virology·K T KaljotH B Greenberg