Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.

PloS One
Yu Han LiuJames Chan

Abstract

Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.

References

Feb 1, 1991·Journal of Neuroscience Research·W F HickeyH Kimura
Jan 1, 1990·Annual Review of Immunology·S S Zamvil, L Steinman
Oct 1, 1995·Clinical Immunology and Immunopathology·R H Swanborg
Mar 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·E N Benveniste
Nov 9, 2000·Journal of Neuroscience Research·S UchidaN Sakuragawa
Jun 14, 2001·Journal of Clinical Immunology·H C von BüdingenC P Genain
Feb 28, 2002·Trends in Immunology·Sarah G HarrisRichard P Phipps
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hans-Christian von BüdingenClaude P Genain
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Craig S ClementsJamie Rossjohn
Dec 16, 2004·Transplantation·Marco BailoOrnella Parolini
Feb 25, 2005·Investigative Ophthalmology & Visual Science·Haochuan LiHassan Alizadeh
Mar 26, 2005·Immunological Reviews·David A HaflerClare Baecher-Allan
Aug 6, 2005·Stem Cells·Toshio MikiStephen C Strom
Sep 13, 2005·Cytokine·Mandy J McGeachy, Stephen M Anderton
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guang-Xian ZhangAbdolmohamad Rostami
Mar 22, 2006·Journal of Neural Transmission·B Engelhardt
Oct 26, 2006·Blood·Eun J GangRita C R Perlingeiro
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew D WoolardJeffrey A Frelinger
May 15, 2007·Biology of Reproduction·Sivakami IlancheranUrsula Manuelpillai
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yasmina LaouarRichard A Flavell
Sep 12, 2008·Current Protocols in Stem Cell Biology·Toshio MikiStephen C Strom
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·James ChanFrank Alderuccio
Apr 25, 2009·Cellular and Molecular Life Sciences : CMLS·Creg J WorkmanDario A A Vignali
Dec 18, 2009·Nature Immunology·Kajsa Wing, Shimon Sakaguchi
Jun 5, 2010·American Journal of Respiratory and Critical Care Medicine·Yuben MoodleyUrsula Manuelpillai
Jun 19, 2010·Nature Reviews. Immunology·Shimon SakaguchiDavid A Hafler
Jun 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongmei LiAbdolmohamad Rostami
Oct 20, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Darwin J Prockop, Joo Youn Oh

❮ Previous
Next ❯

Citations

Sep 6, 2014·Stem Cell Research & Therapy·Courtney A McDonaldTimothy Jm Moss
Jan 14, 2016·Stem Cell Research & Therapy·Toshio MikiBrendan H Grubbs
May 9, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K AnamT A Davis
Apr 5, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bruna R SousaRodrigo R Resende
Nov 8, 2015·Methods : a Companion to Methods in Enzymology·Sean V MurphyAnthony Atala
Aug 27, 2014·Cytotherapy·Dandan ZhuRebecca Lim
Mar 28, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Sabrina GiacoppoEmanuela Mazzon
Jul 6, 2013·The Journal of International Medical Research·Ying LiuHong-Feng Zhuang
Jun 30, 2018·American Journal of Reproductive Immunology : AJRI·Toshio Miki
Jun 2, 2018·Expert Review of Hematology·Luciana TeofiliOrnella Parolini
Jun 29, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Aleksander SkardalShay Soker
Dec 28, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabio MorandiRoberto Gramignoli
May 27, 2020·Stem Cells Translational Medicine·Anna CargnoniOrnella Parolini
Mar 23, 2018·Cell Transplantation·Marta MagattiOrnella Parolini
Jun 11, 2020·Current Diabetes Reports·Charles-Henri Wassmer, Ekaterine Berishvili
Nov 6, 2015·Frontiers in Bioengineering and Biotechnology·Antonietta R SiliniOrnella Parolini
Dec 7, 2016·Frontiers in Cell and Developmental Biology·Clara Di GermanioBarbara Barboni
Oct 18, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Reas S KhanKenneth S Shindler
Jan 23, 2021·International Journal of Molecular Sciences·Quan-Wen LiuHong-Bo Xin
Oct 17, 2020·Stem Cell Research & Therapy·Qiuwan Zhang, Dongmei Lai
Oct 23, 2020·International Journal of Molecular Sciences·Chen QiuJinying Li
Aug 17, 2021·Frontiers in Immunology·Dragana Odobasic, Stephen R Holdsworth
Jun 18, 2019··Yingxuan ShiChengxiang Dai

❮ Previous
Next ❯

Methods Mentioned

BETA
cesarean section
flow cytometry
ELISA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.