PMID: 701356Sep 1, 1978Paper

Amniotic fluid fibronectin. Characterization and synthesis by cells in culture

The Journal of Cell Biology
E CrouchP Bornstein

Abstract

A glycoprotein immunologically related to plasma cold-insoluble globulin (CIG) and fetal skin fibroblast fibronectin has been purified from second-trimester human amniotic fluid. This protein (amniotic fluid fibronectin) migrated more slowly than CIG on sodium dodecyl sulfate gel electrophoresis and showed greater polydispersity which could result, at least in part, from heterogeneity in glycosylation. Cloned human amniotic fluid epithelioid and fibroblastic cells synthesized and secreted a protein with similar properties into the culture medium. Fibronectin was shown to be associated with the pericellular and extracellular matrix of cultured amniotic fluid cells by immunofluorescence, lactoperoxidase-catalyzed iodination, and labeling with ferritin-conjugated antibodies. The kinetics of secretion of the protein were consistent with its role as a matrix protein. We anticipate that amniotic fluid fibronectin will prove to be the same protein which elsewhere in the body is incorporated into connective tissues and basement membranes. Amniotic fluid could, therefore, serve as a convenient source of in vivo synthesized fibronectin for biological and structural studies.

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Citations

Jan 1, 1979·Journal of Supramolecular Structure·S J Fisher, R A Laine
Jan 1, 1979·Journal of Supramolecular Structure·G BalianP Bornstein
Oct 31, 1984·Revista clínica española·M C Llena PuyG Guillén Martínez
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Jan 1, 1981·Collagen and Related Research·E RuoslahtiE G Hayman
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E CrouchP Bornstein
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S ChandrasekharA J Millis
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Aug 30, 2019·Reproduction in Domestic Animals = Zuchthygiene·Gabriela Ferreira Tavares BoyEunice Oba

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