Amniotic membrane as novel scaffold for human iPSC-derived cardiomyogenesis

In Vitro Cellular & Developmental Biology. Animal
Shagufta ParveenPawan Kumar Gupta

Abstract

Recent approaches of using decellularized organ matrices for cardiac tissue engineering prompted us to culture human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) on the human amniotic membrane (hAM). Since hAM has been used lately to patch diseased hearts in patients and has shown anti-inflammatory and anti-fibrotic benefits, it qualifies as a cardiac compatible and clinically relevant heart tissue scaffold. The aim of this study was to test the ability of the hAM to support attachment, differentiation, and maturation of hiPSC-derived CMs in vitro. hAMs were prepared from term placenta. An in-house generated hiPSC line was used for CM derivation. hiPSC-derived cardiac progenitors were cultured on the surface of cryopreserved hAMs and in the presence of cytokines promoting cardiac differentiation. CMs grown on hAM and popular basement membrane matrix (BMM) Matrigel™ were compared for the following aspects of cardiac development: the morphology of cardiomyocytes with respect to shape and cellular alignments, levels of cardiac-related gene transcript expression, functionality in terms of spontaneous calcium fluxes and mitochondrial densities and distributions. hAM is biocompatible with hiPSC-derived CMs. hAM ...Continue Reading

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Citations

Sep 4, 2020·International Journal of Molecular Sciences·Giulia GaggiBarbara Ghinassi
Oct 25, 2020·Stem Cell Reviews and Reports·Pallavi PushpMukesh Kumar Gupta
Oct 1, 2020·Antioxidants & Redox Signaling·Kaveh RoshanbinfarFelix B Engel
Mar 13, 2021·Biomaterials Science·Bahareh Kheilnezhad, Afra Hadjizadeh
Feb 20, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Willem J de LangeJ Carter Ralphe
Jun 3, 2021·Membranes·Mathilde FénelonFlorelle Gindraux

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Datasets Mentioned

BETA
AB45932

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

Adobe Photoshop
Q Capture Pro
ImageJ

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