Amniotic membrane as part of a skin substitute for full-thickness wounds: an experimental evaluation in a porcine model

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Denys John LoeffelbeinMarco R Kesting

Abstract

We evaluated the use of human amniotic membrane (HAM) as a graft material for the treatment of iatrogenic full-thickness (FT) skin wounds in a porcine model with a view to reducing donor site morbidity in free flap transfer. Forty experimental FT-wounds were covered with an autologous split-thickness skin graft (STSG) alone or in combination with a mono- or multilayer HAM or Integra(®). Untreated wounds served as controls. Clinical evaluation and biopsy-sampling for histological and immunohistochemical staining with von-Willebrand-factor (vWF) antibody, laminin antibody, Ki-67 antibody, and smooth muscle actin (αSMA) antibody were performed on days 5, 7, 10, 20, 40, and 60 after surgical intervention. Considerable disparities in the estimated criteria were observed between the various treatment groups of the FT-wounds. The use of HAM was found to have an accelerating impact on re-epithelialization. The multilayered amnion membrane showed better results than the Integra(®) and monolayer technique in terms of contraction rate, inflammation, and scarring and seemed useful as a dermal substitute in FT-wounds giving comparable results to STSG coverage alone. This study demonstrates the successful application of HAM as part of a skin...Continue Reading

References

May 31, 1980·Lancet·J P BennettW P Faulk
May 31, 1980·Lancet·W P FaulkB L Hsi
Jan 1, 1981·International Review of Cytology·C S Potten
Jan 1, 1994·The Journal of Investigative Dermatology·H E KnaggsW J Cunliffe
Jan 1, 1997·Dermatology : International Journal for Clinical and Investigative Dermatology·T ShimizuC Asagami
Jan 30, 1999·The Journal of Pathology·M P AndriessenJ Schalkwijk
May 18, 2000·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Y YamaguchiS Itami
Jun 6, 2000·Advanced Drug Delivery Reviews·A G MikosJ E Babensee
Feb 15, 2001·Plastic and Reconstructive Surgery·C AndreeE Eriksson
Mar 10, 2001·Biochemistry. Biokhimii︠a︡·T G KanyshkovaG A Nevinsky
Jul 1, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Abdoelwaheb El Ghalbzouri, Maria Ponec
Sep 15, 2004·American Journal of Ophthalmology·Tsuyoshi YoshitaScheffer C G Tseng
Sep 30, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Daniel DrueckeLars Steinstraesser
Jan 22, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alexis DesmoulièreGiulio Gabbiani
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Marcia L UsuiJohn E Olerud
Jan 3, 2006·International Journal of Oral and Maxillofacial Surgery·M RinastitiW Sosroseno
May 24, 2007·Tissue Engineering·Kazuyoshi IijimaOsamu Nishizawa
Jun 20, 2007·Experimental Dermatology·Mara FumagalliCarlotta Castagnoli
Jul 26, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Isabelle MermetFrançois Aubin
Nov 22, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michael J ReidJustin R Sharpe
Oct 7, 2008·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Mohammed Ali AkhavaniNorbert Kang
Oct 14, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Marco Rainer KestingLars Steinstraesser
Oct 29, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·JianFei WangUNKNOWN Wound Healing Research Group
Jan 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Cecelia C YatesAlan Wells
May 15, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marco Rainer KestingLars Steinstraesser
May 30, 2009·The British Journal of Oral & Maxillofacial Surgery·Marco Rainer KestingLars Steinstraesser
Aug 26, 2009·International Journal of Dermatology·Venetia Lo, Elena Pope
Oct 30, 2009·Burns : Journal of the International Society for Burn Injuries·Xue-Qing WangRoy M Kimble
Jul 20, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Carmen L InsaustiFrancisco J Nicolás
Jan 25, 2011·Journal of Burn Care & Research : Official Publication of the American Burn Association·Abul K Mostaque, Khairul B M Abdur Rahman

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Citations

Jan 21, 2014·Journal of Pediatric Surgery·Erin G BrownDiana L Farmer
Jul 9, 2014·BioMed Research International·Denys J LoeffelbeinMarco R Kesting
May 28, 2014·Acta Cirúrgica Brasileira·Ian Göedert Leite Duarte, Ivana Duval-Araujo
Feb 15, 2014·Advances in Wound Care·Justin R Sharpe, Yella Martin
Sep 29, 2018·The Journal of Dermatological Treatment·Christina DaiAmor Khachemoune
Jan 17, 2020·Plastic and Reconstructive Surgery. Global Open·Samuel JohnAchim von Bomhard
Oct 13, 2020·Cell Transplantation·Dam Thi Phuong LanPham Van Tran
May 24, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Mengsheng SongYabin Zhu
Apr 4, 2018·ACS Applied Materials & Interfaces·Huanhuan LiuHai-Quan Mao
Oct 29, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Douglas W HamiltonLauren E Flynn

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