Amoebae in Chronic, Polymicrobial Endodontic Infections Are Associated with Altered Microbial Communities of Increased Virulence

Journal of Clinical Medicine
Garrit KollerFrancesco Mannocci

Abstract

Infections of the root canal space involve polymicrobial biofilms and lead to chronic, low grade inflammatory responses arising from the seeding of microbes and by-products. Acute exacerbation and/or disseminating infections occur when established microbial communities undergo sudden changes in phenotypic behaviour. Within clinical endodontic infections, we assessedcategorical determinants comprising, and changing microbial composition of, chronic polymicrobial infections and their association with amoebae. After standardised assessment, primary or secondary infections underwent sampling and DNA processing, targeting bacteria, fungi and amoebae, including 16S high-throughput sequencing. After taxonomic assignment, community composition was correlated with clinical signs and symptoms. Diversity and abundance analyses were carried out in relation to the presence of non-bacterial amplicons. Clinical specimens revealed two distinct community clusters, where specific changes correlated with clinical signs. An association between the compositions of microbiomes was found between these groups and the presence of Entamoeba gingivalis in 44% of cases. When amoebae were present in endodontic infections, we demonstrate changes in microbia...Continue Reading

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Citations

Jun 10, 2021·Journal of Oral Microbiology·Christen Rune StensvoldHenrik Vedel Nielsen

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Methods Mentioned

BETA
Polymerase Chain Reaction
PCR
electrophoresis

Software Mentioned

STAMP
SPSS
hclust
Linear Discriminant Analysis Effect Size ( LEfSe
PermDisp
EstimateS
R Studio
MNDS
R
phyloseq

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