Among multiple phosphomannomutase gene orthologues, only one gene encodes a protein with phosphoglucomutase and phosphomannomutase activities in Thermococcus kodakaraensis

Journal of Bacteriology
Naeem RashidTadayuki Imanaka

Abstract

Four orthologous genes (TK1108, TK1404, TK1777, and TK2185) that can be annotated as phosphomannomutase (PMM) genes (COG1109) have been identified in the genome of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. We previously found that TK1777 actually encodes a phosphopentomutase. In order to determine which of the remaining three orthologues encodes a phosphoglucomutase (PGM), we examined the PGM activity in T. kodakaraensis cells and identified the gene responsible for this activity. Heterologous gene expression and purification and characterization of the recombinant protein indicated that TK1108 encoded a protein with high levels of PGM activity (690 U mg(-1)), along with high levels of PMM activity (401 U mg(-1)). Similar analyses of the remaining two orthologues revealed that their protein products exhibited neither PGM nor PMM activity. PGM activity and transcription of TK1108 in T. kodakaraensis were found to be higher in cells grown on starch than in cells grown on pyruvate. Our results clearly indicate that, among the four PMM gene orthologues in T. kodakaraensis, only one gene, TK1108, actually encodes a protein with PGM and PMM activities.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1994·Journal of Bacteriology·J P YuW B Whitman
Oct 4, 2000·Nature Biotechnology·K E NelsonC M Fraser
Jun 18, 2002·The Journal of Biological Chemistry·Naeem RashidTadayuki Imanaka
Nov 12, 2002·Journal of Bacteriology·Claire M FraserSteven L Salzberg
Apr 7, 2005·Archaea : an International Microbiological Journal·Haruyuki AtomiTadayuki Imanaka

❮ Previous
Next ❯

Citations

Dec 22, 2009·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim
Apr 8, 2010·Glycobiology·Doron CaloJerry Eichler
Feb 12, 2008·Journal of Bacteriology·Seema C Namboori, David E Graham
May 8, 2013·Journal of Bioscience and Bioengineering·Shahid Mahmood Chohan, Naeem Rashid
Nov 25, 2011·Enzyme and Microbial Technology·Elisavet KourtoglouPaul Christakopoulos
Jun 23, 2006·FEMS Microbiology Letters·Harmen J G van de WerkenJohn van der Oost
Apr 15, 2017·Biotechnology and Bioengineering·Chun YouYi-Heng Percival Zhang
Jun 11, 2017·Applied and Environmental Microbiology·Tomoko FujisawaHaruyuki Atomi
Nov 15, 2019·Metabolic Engineering·Kerstin PetrollAnwar Sunna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.