PMID: 11605986Oct 19, 2001Paper

Amount and redox state of cytoplasmic, membrane and periplasmic proteins in Escherichia coli redox mutants

Research in Microbiology
A de Crouy-Chanel, Gilbert Richarme

Abstract

We analyzed the amount and redox state of cytoplasmic, membrane and periplasmic proteins in Escherichia coli mutants deficient in thioredoxin, thioredoxin reductase, glutathione and DsbA, by observing the electrophoretic profile of bacterial extracts after in vivo labelling with monobromobimane. Our results show that these mutations affected not only the amount and the redox state of proteins localized in the same compartment as the deficient oxidoreductase, but also those of the proteins localized in other compartments. These results concord with the hypothesis that there is a link between the redox reactions that occur in the cytoplasm and the periplasm.

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Citations

Jul 29, 2008·Biochemical and Biophysical Research Communications·Fatoum KthiriGilbert Richarme
Aug 10, 2017·Antioxidants & Redox Signaling·Agnès Delaunay-MoisanMichel B Toledano
Jul 26, 2005·The Journal of Biological Chemistry·Marc S PittmanRobert K Poole
Nov 7, 2018·Cold Spring Harbor Perspectives in Biology·Ryo Ushioda, Kazuhiro Nagata

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