Amount and source of dietary copper affects small intestine morphology, duodenal lipid peroxidation, hepatic oxidative stress,and mRNA expression of hepatic copper regulatory proteins in weanling pigs

Journal of Animal Science
R S FryJ W Spears

Abstract

Thirty weanling, crossbred barrows (SUS SCROFA) were used to determine the effects of amount and source of dietary Cu on small intestinal morphology and lipid peroxidation, Cu metabolism, and mRNA expression of proteins involved in hepatic Cu homeostasis. At 21 d of age, pigs were stratified by BW (6.33 ± 0.23 kg) and allocated to 1 of the following dietary treatments: i) control (no supplemental Cu; 6.7 mg Cu/kg), ii) 225 mg supplemental Cu/kg diet from Cu sulfate (CuSO(4)), or iii) 225 mg supplemental Cu/kg diet from tribasic Cu chloride (TBCC). Pigs were housed 2 pigs per pen and were fed a 3-phase diet regimen until d 35 or 36 of the study. During harvest, bile and liver were obtained for mineral analysis, and liver samples were also obtained for analysis of liver glutathione (GSH) and mRNA expression of Cu regulatory proteins. Segments of duodenum, proximal jejunum, and ileum were obtained for mucosal morphology, and duodenal mucosal scrapings were collected from all pigs for analysis of malondialdehyde (MDA). Duodenal villus height was reduced in CuSO(4) pigs compared with control (P = 0.001) and TBCC (P = 0.03) pigs. Villus height in the proximal jejunum of CuSO(4) pigs was reduced (P = 0.03) compared with control pigs, ...Continue Reading

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Citations

Mar 13, 2016·Biochemical and Biophysical Research Communications·Xiaoyan QiangYubin Zhang
Apr 16, 2015·Critical Reviews in Food Science and Nutrition·O B OlafisoyeO A Osibote
Dec 17, 2019·Journal of Animal Physiology and Animal Nutrition·Yang GaoLianyu Yang
Apr 12, 2020·Journal of Animal Science·Sandra Villagómez-EstradaDavid Solà-Oriol
Dec 13, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Alessia Di GiancamilloCarlo Corino
Jul 8, 2018·Biological Trace Element Research·Yaping TianHeyuan Wang
Mar 29, 2019·Frontiers in Veterinary Science·Marcia Carlson Shannon, Gretchen Myers Hill
Jan 7, 2019·Biological Trace Element Research·Gretchen Myers Hill, Marcia Carlson Shannon
Feb 13, 2020·Animals : an Open Access Journal From MDPI·Jinbiao ZhaoChanghua Lai
Apr 8, 2019·Translational Animal Science·Sandra J A Van KuijkYanming Han
Dec 12, 2018·Applied Microbiology and Biotechnology·Feng ZhangWen Yao
Feb 16, 2021·Journal of Animal Science·Juliana RanchesJohn D Arthington

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