Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon

PloS One
Andreas MaderMadeleine Opitz

Abstract

The production of bacteriocins in response to worsening environmental conditions is one means of bacteria to outcompete other microorganisms. Colicins, one class of bacteriocins in Escherichia coli, are effective against closely related Enterobacteriaceae. Current research focuses on production, release and uptake of these toxins by bacteria. However, little is known about the quantitative aspects of these dynamic processes. Here, we quantitatively study expression dynamics of the Colicin E2 operon in E. coli on a single cell level using fluorescence time-lapse microscopy. DNA damage, triggering SOS response leads to the heterogeneous expression of this operon including the cea gene encoding the toxin, Colicin E2, and the cel gene coding for the induction of cell lysis and subsequent colicin release. Advancing previous whole population investigations, our time-lapse experiments reveal that at low exogenous stress levels all cells eventually respond after a given time (heterogeneous timing). This heterogeneous timing is lost at high stress levels, at which a synchronized stress response of all cells 60 min after induction via stress can be observed. We further demonstrate, that the amount of colicin released is dependent on cel ...Continue Reading

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Citations

Mar 14, 2016·Trends in Microbiology·Nadine FornelosMatej Butala
Mar 16, 2017·BMC Evolutionary Biology·Hanna KinnulaLotta-Riina Sundberg
May 15, 2018·Physical Biology·Benedikt von BronkMadeleine Opitz
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Apr 27, 2018·Scientific Reports·Alexandra GötzMadeleine Opitz
Oct 31, 2018·Physical Biology·Benedikt von BronkMadeleine Opitz
Dec 16, 2016·PLoS Computational Biology·Matthias LechnerErwin Frey

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Datasets Mentioned

BETA
M29885

Methods Mentioned

BETA
fluorescence microscopy
PCR
Fluorescence

Software Mentioned

micromanager
Adobe CS5
ImageJ
IgorPRO

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