PMID: 9181305Jun 1, 1997Paper

Amount of the two major Ag-NOR proteins, nucleolin, and protein B23 is cell-cycle dependent

Cytometry
V SirriD Hernandez-Verdun

Abstract

To know the biological basis allowing the use of Ag-NOR protein expression as proliferation marker in human malignancies, the relationship between cell cycle and amount of Ag-NOR protein was analyzed. The quantification of the two major Ag-NOR proteins, nucleolin and protein B23, was performed in exponentially growing, serum-deprived, and cell-cycle stimulated cells. Expression of nucleolin was low in serum-deprived cells and increased mostly in S phase during cell-cycle stimulation. Conversely, expression of protein B23 was slightly repressed in serum-deprived cells, and increased progressively until G2 phase during cell-cycle stimulation. The accumulation of nucleolin and protein B23 in G2 compared to G1 was demonstrated using sorted phase-specific cells. In G0, cells sorted according to their very low RNA content, and the amount of Ag-NOR proteins was half of that found in G1 cells, nucleolin being only weakly detectable. Therefore, the expression of nucleolin increased between G0-G1 and G1-S phases. These data support the hypothesis that quantification of Ag-NOR proteins is an estimation of the percentage of cells in each cell cycle phase because their amount is high in S-G2 and low in G1 phases.

Citations

Apr 23, 2002·Journal of Virology·Hongying ChenJulian A Hiscox
Sep 7, 2006·Molecular and Cellular Biology·Vanesa MuncanAlan R Clarke
Nov 30, 2007·Histochemistry and Cell Biology·Valentina SirriDanièle Hernandez-Verdun
Mar 7, 2008·Journal of Virology·Aleth CalléAnna Greco
May 4, 2011·International Journal of Medical Sciences·Feng-Xian QinLing Zhang
Feb 13, 2016·Genetic Testing and Molecular Biomarkers·Sener TasdemirHasan Turkez
Nov 27, 2007·Morphologie : Bulletin De L'Association Des Anatomistes·F Pascaretti-GrizonD Chappard
Sep 21, 2006·Cancer Science·Tomoki NaoeTakeshi Urano
Aug 30, 2014·Cellular & Molecular Biology Letters·Dorota Hoja-ŁukowiczAnna Lityńska
Apr 29, 2009·Reviews in Medical Virology·Anna Greco
Jan 26, 1999·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Apr 12, 2011·Folia Histochemica Et Cytobiologica·Tomasz FerencJacek Sygut
Nov 5, 2011·AIDS Research and Human Retroviruses·Giuseppa VisalliGiuseppe Piedimonte
Nov 30, 2007·Cytometry. Part B, Clinical Cytometry·Zuhal HamurcuSefer Kumandas
Oct 12, 2005·Histochemistry and Cell Biology·Fernando González-Camacho, Francisco Javier Medina
Jun 15, 2007·The Journal of Biological Chemistry·Ming XieHidetaka Sakai

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