AMP-activated protein kinase activation by adrenoceptors in L6 skeletal muscle cells: mediation by alpha1-adrenoceptors causing glucose uptake

Diabetes
Dana S Hutchinson, Tore Bengtsson

Abstract

AMP-activated protein kinase (AMPK), which functions as a sensor of cellular energy homeostasis, was phosphorylated after norepinephrine stimulation in L6 skeletal muscle cells. This effect was mediated by alpha1-adrenoceptors, with no stimulatory effects due to interactions at alpha2- or beta-adrenoceptors. Alpha1-adrenoceptors are Gq-coupled receptors, and calcium but not phorbol esters could mimic the effect of alpha1-adrenergic stimulation; and we show that protein kinase C is not involved as an upstream signal to AMPK by alpha1-adrenergic stimulation and that the AMP-to-ATP ratio is unaltered after alpha1-adrenergic stimulation. We further show that glucose uptake mediated by alpha1- but not by beta-adrenoceptors can be inhibited by AMPK inhibition. Acetyl-CoA carboxylase (ACC) is phosphorylated at Ser218 by AMPK, and alpha1- but not beta-adrenoceptor stimulation results in phosphorylation of ACC at this residue. These results suggest a novel pathway where alpha1-adrenoceptor activation, independent of protein kinase C, leads to activation of AMPK in skeletal muscle, which contributes to alpha1-adrenoceptor-mediated increases in glucose uptake.

References

May 14, 1986·Biochemical and Biophysical Research Communications·S RattiganE A Richter
Feb 1, 1995·British Journal of Pharmacology·Y L Liu, M J Stock
Jun 1, 1993·British Journal of Pharmacology·S J RobertsR J Summers
Jun 4, 1996·Biochimica Et Biophysica Acta·N HeninG Van den Berghe
Aug 29, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·J T Cheng, I M Liu
Sep 28, 2000·Biochemical and Biophysical Research Communications·K KishiY Ebina
Jan 18, 2002·Nature·Yasuhiko MinokoshiBarbara B Kahn
May 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Hideyuki SakodaTomoichiro Asano
Aug 17, 2002·British Journal of Pharmacology·Julia NevzorovaRoger J Summers
Nov 21, 2002·Biochemical Society Transactions·M R Munday
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eva TomasNeil B Ruderman
Aug 28, 2003·The Journal of Biological Chemistry·Wu YinMorris J Birnbaum
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Reuben J ShawLewis C Cantley
Oct 29, 2004·Journal of Cell Science·D Grahame Hardie
May 10, 2005·The Journal of Biological Chemistry·Marie DavalFabienne Foufelle
Jun 28, 2005·The Journal of Biological Chemistry·Rebecca L HurleyLee A Witters

❮ Previous
Next ❯

Citations

Apr 9, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Robert A NgalaJonathan R S Arch
Jul 27, 2007·Cell Biochemistry and Biophysics·William W Winder, David M Thomson
Nov 30, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Marie E GibbsRoger J Summers
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ji LiLawrence H Young
Jan 7, 2011·The Journal of Biological Chemistry·Thomas PulinilkunnilBarbara B Kahn
Jul 24, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Masahiro IwataShigeyuki Suzuki
Nov 12, 2014·The Journal of Cell Biology·Jessica M OlsenTore Bengtsson
Jan 17, 2016·Journal of Molecular and Cellular Cardiology·Evangelos P DaskalopoulosSandrine Horman
Oct 27, 2007·The Journal of Physiology·Jonas Møller KristensenJørgen F P Wojtaszewski
Apr 15, 2011·Trends in Endocrinology and Metabolism : TEM·Joseph S MarinoJennifer W Hill
Sep 3, 2011·British Journal of Pharmacology·Nodi DehvariTore Bengtsson
Feb 7, 2012·Biochemical and Biophysical Research Communications·Na-Young HongDeok-Bae Park
Jan 22, 2013·Cellular Signalling·Alberto OuroAntonio Gomez-Muñoz
Jun 10, 2016·Journal of Receptor and Signal Transduction Research·Ting ShiDianne M Perez
Mar 12, 2016·Experimental & Molecular Medicine·Eun RohMin-Seon Kim
May 22, 2009·American Journal of Physiology. Endocrinology and Metabolism·Thomas E JensenErik A Richter
Dec 14, 2017·Pharmacological Reviews·Darren M RiddyChristopher J Langmead
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jagdip S JaswalAlexander S Clanachan
Mar 21, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Saori MukaidaTore Bengtsson
Oct 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S SummermatterA G Dulloo
Jul 25, 2019·Molecular Neurobiology·Vijay Sankar RamasamyJihoon Jo
Aug 8, 2020·Journal of Receptor and Signal Transduction Research·Robert S Papay, Dianne M Perez
Apr 27, 2017·British Journal of Pharmacology·Edilson Dantas da Silva JuniorRoger J Summers
Nov 20, 2020·Scientific Reports·Yashodani PillayAnil A Chuturgoon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.