AMP-activated protein kinase (AMPK) is activated in muscle of subjects with type 2 diabetes during exercise

Diabetes
Nicolas MusiL J Goodyear

Abstract

Insulin-stimulated GLUT4 translocation is impaired in people with type 2 diabetes. In contrast, exercise results in a normal increase in GLUT4 translocation and glucose uptake in these patients. Several groups have recently hypothesized that exercise increases glucose uptake via an insulin-independent mechanism mediated by the activation of AMP-activated protein kinase (AMPK). If this hypothesis is correct, people with type 2 diabetes should have normal AMPK activation in response to exercise. Seven subjects with type 2 diabetes and eight matched control subjects exercised on a cycle ergometer for 45 min at 70% of maximum workload. Biopsies of vastus lateralis muscle were taken before exercise, after 20 and 45 min of exercise, and at 30 min postexercise. Blood glucose concentrations decreased from 7.6 to 4.77 mmol/l with 45 min of exercise in the diabetic group and did not change in the control group. Exercise significantly increased AMPK alpha2 activity 2.7-fold over basal at 20 min in both groups and remained elevated throughout the protocol, but there was no effect of exercise on AMPK alpha1 activity. Subjects with type 2 diabetes had similar protein expression of AMPK alpha1, alpha2, and beta1 in muscle compared with contro...Continue Reading

References

Oct 1, 1988·Clinical Physiology·L Wallin, L H Brudin
May 1, 1984·Critical Care Medicine·L C ClarkM S Moore
Sep 1, 1995·The American Journal of Physiology·I K MartinJ Wahren
Jul 11, 1994·The American Journal of Physiology·J T BrozinickJ L Ivy
Aug 1, 1993·The American Journal of Physiology·P A KingE S Horton
Jan 12, 1996·The Journal of Biological Chemistry·D StapletonB E Kemp
Jan 1, 1996·Reviews of Physiology, Biochemistry and Pharmacology·J O Holloszy, P A Hansen
Oct 1, 1996·Diabetologia·M F VincentG Van den Berghe
May 16, 1997·The Journal of Biological Chemistry·D VavvasN B Ruderman
Oct 24, 1997·Journal of Applied Physiology·B B Rasmussen, W W Winder
Mar 24, 1998·Annual Review of Medicine·L J Goodyear, B B Kahn
Oct 6, 1998·Annual Review of Biochemistry·D G HardieM Carlson
Jan 14, 1999·The American Journal of Physiology·N B RudermanL A Witters
Mar 24, 1999·Trends in Biochemical Sciences·B E KempL A Witters
May 18, 1999·The American Journal of Physiology·R BergeronG I Shulman
Jul 17, 1999·The American Journal of Physiology·W W Winder, D G Hardie
Jul 22, 1999·The New England Journal of Medicine·P R Shepherd, B B Kahn
Aug 13, 1999·The American Journal of Physiology·J IhlemannH Galbo
Aug 13, 1999·The American Journal of Physiology·R R RussellL H Young
Jul 13, 2000·Biochemical and Biophysical Research Communications·N FujiiL J Goodyear
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·Z P ChenB E Kemp

❮ Previous
Next ❯

Citations

Dec 18, 2001·Diabetes/metabolism Research and Reviews·B NazlielB Koçer
Aug 25, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhaosheng TangLian Zhu
Jun 9, 2005·European Journal of Applied Physiology·Taketaka HaraShigeo Fujimoto
Mar 23, 2004·Biochemical and Biophysical Research Communications·Aina S LihnBjørn Richelsen
Mar 14, 2002·Molecular Aspects of Medicine·G A Rutter
Dec 7, 2002·Neuromuscular Disorders : NMD·M Orth, R R Mundegar
Apr 26, 2003·Trends in Endocrinology and Metabolism : TEM·Núria Morral
Nov 12, 2010·The Proceedings of the Nutrition Society·D Grahame Hardie
Oct 26, 2005·International Journal of Obesity : Journal of the International Association for the Study of Obesity·T MuraseI Tokimitsu
Aug 6, 2004·The Proceedings of the Nutrition Society·Nobuharu FujiiLaurie J Goodyear
May 8, 2008·Current Opinion in Lipidology·Edward W Kraegen, Gregory J Cooney
Nov 1, 2007·Diabetes, Obesity & Metabolism·S T JohnsonN G Boulé
Feb 22, 2012·Diabetes, Obesity & Metabolism·S E HusseyM Hargreaves
Feb 28, 2009·Acta Physiologica·B D HegartyE W Kraegen
Feb 28, 2009·Acta Physiologica·N L Dzamko, G R Steinberg
Mar 3, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Camilla Skov-JensenFlemming Dela
May 19, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Henrik GalboLuc J C van Loon
Apr 21, 2011·American Journal of Physiology. Endocrinology and Metabolism·Niels JessenLaurie J Goodyear
Feb 5, 2009·American Journal of Physiology. Endocrinology and Metabolism·John Andree BabrajDaniel James Cuthbertson
Aug 31, 2006·BMC Bioinformatics·Qingfeng Chen, Yi-Ping Phoebe Chen
Mar 9, 2013·Journal of Lipid Research·Jonathan Dalla-RivaJens O Lagerstedt
Mar 15, 2006·Medicine and Science in Sports and Exercise·Adam L MacDonaldPeter W Watt
Jul 11, 2008·Medicine and Science in Sports and Exercise·Robert S Lee-YoungGlenn K McConell
Nov 19, 2010·Medicine and Science in Sports and Exercise·Sheri R ColbergUNKNOWN American Diabetes Association
Nov 16, 2013·PloS One·Zar Chi ThentLeonard Joseph Henry
Sep 10, 2011·Journal of the American Society of Nephrology : JASN·Qifei HanYoufei Guan
Feb 18, 2004·Sports Medicine·William G AschenbachLaurie J Goodyear
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Benoit ViolletFabrizio Andreelli
Feb 20, 2014·Sports Medicine·Bjorn T Tam, Parco M Siu
Apr 3, 2008·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Greg D WellsIngrid Tein

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.