AMP-activated protein kinase is involved in perfluorohexanesulfonate-induced apoptosis of neuronal cells

Chemosphere
Youn Ju LeeJae-Ho Yang

Abstract

Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds (PFCs), has been used in a variety of industrial and consumer applications and detected in serum in the general population. This raised a concern over its possible detrimental health effects, including neurotoxic effects. We have previously shown that PFHxS induced neuronal apoptosis via the NMDA receptor-mediated extracellular signal-regulated kinase (ERK) pathway. Recently, it has been reported that AMP-activated protein kinase (AMPK) acts as a key signal molecule in neuronal excitotoxicity as well as providing a neuroprotective function. In the present study, we have examined the involvement of AMPK in PFHxS-induced neuronal apoptosis using neuronal differentiated PC12 cells. PFHxS induced significant increases in intracellular [Ca(2+)] via the NMDA receptor and the L-type voltage-gated calcium channel (L-VGCC). The inhibition of Ca(2+) loading by the NMDA receptor antagonist, MK801 and the L-VGCC blockers, nifedipine and diltiazem significantly reduced PFHxS-induced apoptosis. PFHxS induced sustained activation of AMPK and the inhibition of AMPK activation by compound C and AMPK siRNA significantly reduced PFHxS-induced caspase-3 activity. These res...Continue Reading

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Citations

Jun 24, 2017·Apoptosis : an International Journal on Programmed Cell Death·A BobbaA Atlante
Oct 18, 2019·Yeungnam University Journal of Medicine·Youn Ju Lee
Feb 18, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Shih-Chang FuYa-Wen Chen

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