AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid

Nutrition & Metabolism
Xihong ZhouYizhen Wang

Abstract

n-3 long chain polyunsaturated fatty acid (n-3 LC PUFA) increases β-oxidation and limits lipid accumulation in adipocytes. The current study was conducted to determine whether their precursor alpha-linolenic acid (ALA) could also exert the above effects and how AMP-activated protein kinase (AMPK) was involved. AMPKα1(-/-), AMPKα2(-/-) mice and wild-type (WT) mice were fed a high-fat diet (HFD) or HFD with ALA. Body weight was recorded weekly and serum was collected. Adipocytes size and expression of key players involved in mitochondrial biogenesis and lipid oxidation were also measured. Our results showed an elevated serum adiponectin level and a decreased leptin and insulin level in WT mice fed HFD with ALA when compared with WT mice fed HFD. In addition, dietary ALA decreased epididymal adiposity and adipocytes size in WT mice. At protein level, mitochondrial genes (peroxisome proliferator-activated receptor gamma coactivator 1 alpha [PGC1α] and nuclear respiratory factor-1 [nrf1]) and β-oxidation related genes (carnitine palmitoyltransferase 1A [CPT1a] and peroxisome proliferator-activated receptor alpha [PPARα]) were upregulated by dietary ALA in epididymal fat of WT mice. Consistently, dietary ALA also increased mitochondr...Continue Reading

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Citations

Jan 11, 2017·Nutrition & Metabolism·Hao-Neng TangHou-De Zhou
Jan 5, 2019·BMC Complementary and Alternative Medicine·Yea-Jin ParkHyo-Jin An
Feb 5, 2021·Redox Biology·M Pilar Bayona-BafaluyEduardo Ruiz-Pesini
Sep 24, 2021·Phytotherapy Research : PTR·Qianghua YuanLi Liu
Nov 29, 2017·Molecular Immunology·Takumi Kobayashi, Stephen R Mattarollo

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