AMP-activated protein kinase is required for induction of apoptosis and epithelial-to-mesenchymal transition

Cellular Signalling
Xunde WangJianguo Song

Abstract

AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase which has been implicated in the regulation of cellular energy homeostasis. Relatively very little is known about its role in other cellular processes. We observed that AMPK-alpha can be activated by transforming growth factor-beta1 (TGF-beta1) in mouse hepatocytes. Inhibition of AMPK by Compound C, a selective AMPK-alpha inhibitor, inhibited TGF-beta1-induced apoptosis and EMT in hepatocytes. In addition, overexpression of a dominant-negative form of AMPK-alpha subunit also suppressed TGF-beta1-induced EMT and apoptosis in AML12 cells. Furthermore, inhibition of AMPK suppressed TGF-beta1-induced Smad3 transcriptional activity. This study indicates that AMPK is able to modulate Smad3 transcriptional activity, which plays an important role in TGF-beta1-induced apoptosis and EMT.

References

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Citations

Apr 16, 2015·International Journal of Molecular Medicine·Xiuli ZhangHongjuan Li
Dec 23, 2015·Biochemical and Biophysical Research Communications·Yue JiaHao Pan
Nov 7, 2016·Journal of Mammary Gland Biology and Neoplasia·Saevar IngthorssonThorarinn Gudjonsson
Feb 7, 2019·Journal of Molecular Cell Biology·Weiwei ShiJianguo Song
Mar 18, 2020·American Journal of Physiology. Endocrinology and Metabolism·Naohiro YanoTing C Zhao
Oct 27, 2017·Acta Biochimica Et Biophysica Sinica·Jianguo Song, Weiwei Shi
Oct 17, 2018·Scientific Reports·Jiayu LiuYuxin Chen
Jan 1, 2014·International Journal of Chronic Diseases·Zoltan MolnarAndrew G Demaine
Jun 7, 2018·Acta Biochimica Et Biophysica Sinica·Jiayu GaoZhijun Luo

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