AMP-guided tumour-specific nanoparticle delivery via adenosine A1 receptor

Biomaterials
Tongcheng DaiQin Liu

Abstract

Active targeting-ligands have been increasingly used to functionalize nanoparticles for tumour-specific clinical applications. Here we utilize nucleotide adenosine 5'-monophosphate (AMP) as a novel ligand to functionalize polymer-based fluorescent nanoparticles (NPs) for tumour-targeted imaging. We demonstrate that AMP-conjugated NPs (NPs-AMP) efficiently bind to and are following internalized into colon cancer cell CW-2 and breast cancer cell MDA-MB-468 in vitro. RNA interference and inhibitor assays reveal that the targeting effects mainly rely on the specific binding of AMP to adenosine A1 receptor (A1R), which is greatly up-regulated in cancer cells than in matched normal cells. More importantly, NPs-AMP specifically accumulate in the tumour site of colon and breast tumour xenografts and are further internalized into the tumour cells in vivo via tail vein injection, confirming that the high in vitro specificity of AMP can be successfully translated into the in vivo efficacy. Furthermore, NPs-AMP exhibit an active tumour-targeting behaviour in various colon and breast cancer cells, which is positively related to the up-regulation level of A1R in cancer cells, suggesting that AMP potentially suits for more extensive A1R-overe...Continue Reading

References

May 7, 2002·International Journal of Cancer. Journal International Du Cancer·David W HoskinJonathan Blay
Jul 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lea MadiPnina Fishman
Mar 2, 2005·Nature Reviews. Cancer·Mauro Ferrari
Mar 7, 2006·Nature Reviews. Drug Discovery·Kenneth A Jacobson, Zhan-Guo Gao
Aug 30, 2008·Trends in Biotechnology·Kathleen F Pirollo, Esther H Chang
Oct 14, 2008·Pharmacology & Therapeutics·Luca AntonioliCorrado Blandizzi
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chung Hang J ChoiMark E Davis
Oct 5, 2010·Biochimica Et Biophysica Acta·Stefania GessiPier Andrea Borea
Apr 26, 2011·Current Drug Discovery Technologies·Samuli HirsjärviJean-Pierre Benoit
Sep 3, 2011·Breast Cancer Research : BCR·Deborah L Holliday, Valerie Speirs
Sep 6, 2011·Annual Review of Medicine·Andrew Z WangOmid C Farokhzad
Sep 29, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Twan LammersGert Storm
May 23, 2012·Current Medicinal Chemistry·M A HolgadoJ L Arias
Jun 28, 2012·Molecular Pharmaceutics·Niladri ChattopadhyayRaymond M Reilly
Mar 9, 2013·Future Oncology·Meidi Hu, Kunhe Zhang
Apr 2, 2013·Angiogenesis·E M ProkopiouJ J Walsh
Sep 27, 2013·Materials Science & Engineering. R, Reports : a Review Journal·Hongmin ChenJin Xie
Nov 12, 2013·Trends in Biotechnology·Gavin T NobleBasar Bilgicer
Nov 15, 2013·Nature Reviews. Cancer·Luca AntonioliGyörgy Haskó
Nov 26, 2013·Advanced Drug Delivery Reviews·Nicolas BertrandOmid C Farokhzad
May 9, 2014·Nature Communications·Ran NamgungWon Jong Kim

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Citations

Jul 30, 2019·Advanced Materials·Peng MiKazunori Kataoka
May 21, 2020·Chemical Communications : Chem Comm·Yun LuoClaire Mangeney
Jan 8, 2017·Biomaterials·Leoni A Kunz-SchughartSuman Pokhrel

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