AMPA receptor-mediated modulation of inward rectifier K+ channels in astrocytes of mouse hippocampus

Molecular and Cellular Neurosciences
Wolfgang SchröderChristian Steinhäuser

Abstract

Astrocytes and neurons are tightly associated and recent data suggest a direct signaling between neuronal and glial cells in vivo. To further analyze these interactions, the patch-clamp technique was combined with single-cell RT-PCR in acute hippocampal brain slices. Subsequent to functional analysis, the cytoplasm of the same cell was harvested to perform transcript analysis and identify subunits that underlie inwardly rectifying K+ currents (I(Kir)) in astrocytes of the CA1 stratum radiatum. Transcripts encoding Kir2.1, Kir2.2, or Kir2.3, were encountered in a majority of cells, while Kir4.1 was less frequent. Further investigation revealed that glial Kir channels are rapidly inhibited upon activation of AMPA-type glutamate receptors, most probably due a receptor-mediated influx of Na+, which plugs the channels from the intracellular side. A transient inhibition of I(Kir) in astrocytes in response to neuronal glutamate release and glial AMPA receptor activation represents a further, so far undetected mechanism to balance neuronal excitability.

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Citations

Aug 12, 2009·Journal of Biological Physics·G RicciG Siciliano
Nov 14, 2008·Stem Cells and Development·Leila HusseiniChristian Steinhäuser
Sep 17, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Simon HöftChristian Steinhäuser
Dec 19, 2009·Brain Research Reviews·Dwight E BerglesChristian Steinhäuser
Oct 16, 2007·Journal of the Neurological Sciences·Jacques De KeyserMarcus W Koch
Mar 8, 2008·Epilepsia·Ronald JabsChristian Steinhäuser
Aug 12, 2008·Journal of Neurochemistry·Michelle L Olsen, Harald Sontheimer
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Nov 25, 2004·Neuroscience·P Kofuji, E A Newman
Sep 17, 2004·Pharmacology & Therapeutics·Raimondo D'Ambrosio
Oct 11, 2005·Molecular and Cellular Neurosciences·Kerstin HüttmannChristian Steinhäuser
Dec 18, 2013·Translational Neuroscience·Angela LanciottiElena Ambrosini
Jul 25, 2006·Molecular and Cellular Neurosciences·Ludmila SosulinaHans-Christian Pape
Apr 29, 2008·Neuron·Jonathon WetheringtonRay Dingledine
Jun 17, 2016·Brain Structure & Function·Behrouz Moshrefi-RavasdjaniChristine R Rose
Mar 23, 2017·Scientific Reports·Damian DrosteChristian Lohr
Jan 6, 2004·Journal of Neuroscience Research·Atsushi TakedaNaoto Oku
Mar 5, 2004·Glia·M L Olsen, H Sontheimer
Jul 15, 2004·Glia·Shih-Chun Lin, Dwight E Bergles
Aug 4, 2006·Glia·Devin K Binder, Christian Steinhäuser
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Mar 2, 2005·Journal of Neurophysiology·Lane K BekarWolfgang Walz
Sep 19, 2007·Glia·Haruki Higashimori, Harald Sontheimer
Feb 24, 2006·Nature Reviews. Neuroscience·Gerald SeifertChristian Steinhäuser
May 21, 2010·Journal of Neurophysiology·Ludmila SosulinaHans-Christian Pape

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