Amperometric choline biosensor based on multiwalled carbon nanotubes/zirconium oxide nanoparticles electrodeposited on glassy carbon electrode

Analytical Biochemistry
S PundirChandra Shekhar Pundir

Abstract

A bienzymatic choline biosensor was constructed by coimmobilizing acetylcholinesterase (AChE) and choline oxidase (ChO) onto nanocomposite of carboxylated multiwalled carbon nanotubes (c-MWCNTs) and zirconium oxide nanoparticles (ZrO(2)NPs) electrodeposited on the surface of a glassy carbon electrode (GCE) and using it (AChE-ChO/c-MWCNT/ZrO(2)NPs/GCE) as working electrode, Ag/AgCl as reference electrode, and Pt wire as auxiliary electrode connected through a potentiostat. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and cyclic voltammetry (CV) studies, optimized, and evaluated. The biosensor exhibited optimum response within 4 s at +0.2V, pH 7.4, and 25 °C. The detection limit and working range of the biosensor were 0.01 μM and 0.05 to 200 μM, respectively. The half-life of the enzyme electrode was 60 days at 4 °C. The serum choline level, as measured by the biosensor, was 9.0 to 12.8 μmol/L (with a mean of 10.81 μmol/L) in apparently healthy persons and 5.0 to 8.4 μmol/L (with a mean of 6.53 μmol/L) in persons suffering from Alzheimer's disease. The enzyme electrode was unaffected by a number of serum substances.

References

Jul 25, 2000·Nutrition·S H Zeisel

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Citations

Jun 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jian-Rong CaiEn Han
Nov 12, 2019·Preparative Biochemistry & Biotechnology·Rüya BaskinFatma Arslan
Jul 6, 2018·Mikrochimica Acta·Jaise Mariya GeorgeBeena Mathew
May 29, 2021·Biosensors & Bioelectronics·Yuqian ZhangAli K Yetisen
Dec 8, 2020·ACS Chemical Neuroscience·Sasya MadhurantakamUma Maheswari Krishnan

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