Amperometric determination of H2O2 at nano-TiO2/DNA/thionin nanocomposite modified electrode

Colloids and Surfaces. B, Biointerfaces
Po-Hsun LoShen-Ming Chen

Abstract

We report electrochemical preparation and characterization of a new biosensor made of nanostructured titanium dioxide (nano-TiO2) particles and deoxyribonucleic acid (DNA). Thionin (TN) redox mediator was electrochemically deposited onto DNA/nano-TiO2 modified glassy carbon electrode (GCE). The X-ray diffraction analysis, atomic force microscope (AFM) and scanning electron microscope (SEM) were used for surface analysis of TN/DNA/nano-TiO2 film. In neutral buffer solution, TN/DNA/nano-TiO2/GCE biosensor exhibited excellent electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) and oxygen (O2). The biosensor shows excellent analytical performance for amperometric determination of H2O2, at reduced overpotential (-0.2V). The detection limit and liner calibration range were found to be 0.05 mM (S/N=3) and 0.05-22.3 mM, respectively. In addition, determination of H2O2 in real samples was carried out using the new biosensor with satisfactory results. The TN/DNA/nano-TiO2/GCE showed stable and reproducible analytical performance towards the reduction of H2O2. This biosensor can be used as an amperometric biosensor for the determination of H2O2 in real samples.

References

Apr 6, 1999·Chemico-biological Interactions·H H LandinS Osterman-Golkar
Sep 8, 2000·Science·T A TatonR L Letsinger
Aug 31, 2002·Science·YunWei Charles CaoChad A Mirkin
Apr 15, 2003·Nature Materials·Tatjana PauneskuGayle Woloschak
Apr 8, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Liping Lu, Xiangqin Lin
Jul 20, 2004·Nature Materials·Q X JiaS R Foltyn
Feb 1, 2005·Biosensors & Bioelectronics·Xiangqin LinLiping Lu
Jul 18, 2006·Nature Materials·Xue-Qing GongUlrike Diebold
Apr 21, 2007·Journal of Inorganic Biochemistry·Xiuli LiXianyu Huang
Nov 21, 2007·Journal of Bioscience and Bioengineering·Chiaki OginoNobuaki Shimizu

❮ Previous
Next ❯

Citations

Nov 28, 2012·International Journal of Biological Macromolecules·Lida FotouhiMajid M Heravi
Mar 1, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Ali A EnsafiHassan Karimi-Maleh
Aug 3, 2010·Colloids and Surfaces. B, Biointerfaces·Roya DastjerdiShadi Shahsavan
Nov 26, 2015·Analytical Chemistry·María Moreno-GuzmánAlberto Escarpa
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Mohammad Mansoob KhanMoo Hwan Cho
Dec 15, 2010·Colloids and Surfaces. B, Biointerfaces·Tao YangKui Jiao
Jan 13, 2017·Journal of Analytical Methods in Chemistry·Giovanni FuscoCristina Tortolini
Jan 1, 2016·Journal of Food and Drug Analysis·Navid NasirizadehMasoumeh Tabatabaee
Sep 17, 2018·Biosensors & Bioelectronics·Dipali R Bagal-KestwalBeen-Huang Chiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.