Amperometric glucose biosensor based on assisted ion transfer through gel-supported microinterfaces

Analytical Chemistry
Carlos M PereiraFernando Silva

Abstract

A novel amperometric glucose sensor was developed based on the facilitated proton transfer across microinterfaces between two immiscible electrolyte solutions. The combination of a 1,3:2,4-dibenzylidene sorbitol/2-nitrophenyl octyl ether gel membrane and 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine as the ionophore allows the transfer of protons from water to the gellified organic phase; the gel membrane is supported on arrays of microholes drilled on a polyester film. The protons are generated as the result of the dissociation of gluconic acid produced during the enzymatic degradation of glucose by glucose oxidase. The characteristics of the glucose sensor were investigated using several experimental conditions, namely, the concentration of ligand and enzyme. The electrochemical response is typical of an enzymatic electrode and displays a linear behavior in the range 0.2-3 mM glucose. The effect of the experimental parameters of the voltammetric technique was also optimized with the aim of improving sensor sensitivity.

Citations

Apr 7, 2006·Analytical and Bioanalytical Chemistry·Ping JingYuanhua Shao
Jun 15, 2006·Analytical Chemistry·Eric Bakker, Yu Qin
Oct 7, 2010·Physical Chemistry Chemical Physics : PCCP·Shaikh Nayeem FaisalHye Jin Lee
Oct 31, 2009·Nanotechnology·Wei-Chi Lai, Shen-Chen Tseng
Jan 27, 2007·The Journal of Organic Chemistry·Eric V Anslyn
Mar 30, 2007·Organic Letters·Tianzhi Zhang, Eric V Anslyn

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