Amperometric glucose biosensor based on adsorption of glucose oxidase at platinum nanoparticle-modified carbon nanotube electrode

Analytical Biochemistry
Hao TangYafei Kuang

Abstract

A new amperometric biosensor, based on adsorption of glucose oxidase (GOD) at the platinum nanoparticle-modified carbon nanotube (CNT) electrode, is presented in this article. CNTs were grown directly on the graphite substrate. The resulting GOD/Pt/CNT electrode was covered by a thin layer of Nafion to avoid the loss of GOD in determination and to improve the anti-interferent ability. The morphologies and electrochemical performance of the CNT, Pt/CNT, and Nafion/GOD/Pt/CNT electrodes have been investigated by scanning electron microscopy, cyclic voltammetry, and amperometric methods. The excellent electrocatalytic activity and special three-dimensional structure of the enzyme electrode result in good characteristics such as a large determination range (0.1-13.5mM), a short response time (within 5s), a large current density (1.176 mA cm(-2)), and high sensitivity (91mA M(-1)cm(-2)) and stability (73.5% remains after 22 days). In addition, effects of pH value, applied potential, electrode construction, and electroactive interferents on the amperometric response of the sensor were investigated and discussed. The reproducibility and applicability to whole blood analysis of the enzyme electrode were also evaluated.

References

Dec 6, 1996·Science·W Z LiG Wang
May 21, 1999·Science·R H BaughmanM Kertesz
Aug 6, 2002·Science·Ray H BaughmanWalt A de Heer
Aug 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuan-Di ZhaoQing-Ming Luo
Feb 27, 2003·Journal of the American Chemical Society·Joseph WangYuehe Lin
May 2, 2003·Analytical Chemistry·Joseph Wang, Mustafa Musameh

❮ Previous
Next ❯

Citations

Apr 2, 2013·Biosensors & Bioelectronics·Viviana Scognamiglio
Nov 26, 2008·Chemical Communications : Chem Comm·Xiaojun ZhangBin Fang
Dec 14, 2011·Sensors·Usman Latif, Franz L Dickert
May 20, 2014·Analytical Biochemistry·Manjusha Mathew, N Sandhyarani
Aug 21, 2012·International Journal of Biological Macromolecules·Khadijeh Eskandari, Hedayatollah Ghourchian
Nov 29, 2014·Nature Reviews. Drug Discovery·Omid VeisehRobert Langer
Apr 27, 2007·Biotechnology Progress·Jithesh V Veetil, Kaiming Ye
Aug 14, 2010·Analytical and Bioanalytical Chemistry·Emer LahiffDermot Diamond
Oct 18, 2005·Analytical and Bioanalytical Chemistry·Jian LiXing-Hua Xia
Mar 29, 2006·Analytical and Bioanalytical Chemistry·Kannan Balasubramanian, Marko Burghard
Mar 2, 2013·Physical Chemistry Chemical Physics : PCCP·Michael E SnowdenPatrick R Unwin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.