Amperometric magnetoimmunosensor for ErbB2 breast cancer biomarker determination in human serum, cell lysates and intact breast cancer cells

Biosensors & Bioelectronics
U EletxigerraS Campuzano

Abstract

A highly sensitive amperometric magnetoimmunosensor for the determination of ErbB2 protein, a well-known biomarker related to high-impact high-incidence diseases such as breast cancer, is described. A sandwich format involving the covalent immobilization of a specific capture antibody onto magnetic beads (MBs) and incubation of the modified MBs with a mixture solution of the antigen and a HRP-labeled detector antibody was used. The resulting modified MBs were captured on the surface of a disposable screen-printed carbon electrode (SPCE) and the amperometric responses at -0.20V were measured. This ErbB2 magnetoimmunosensor exhibited a very low detection limit of 26pgmL(-)(1) far below the established cut-off for this biomarker (15ngmL(-1)) and was successfully applied to the quantitation of ErbB2 in human serum and cell lysates samples without any matrix effect. In addition, the developed assay allowed the assessment of ErbB2 status directly in intact breast cancer cells. The results correlated well with those obtained with a commercial ELISA method, thus demonstrating that the new magnetoimmunosensing platform offers a truthful and useful analytical tool to be easily applied in breast cancer diagnosis through either ErbB2 prote...Continue Reading

References

Aug 17, 2014·Talanta·Raquel C B MarquesM Begoña González-García

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Citations

Dec 9, 2017·Journal of Cellular Physiology·Seyed Hamed JafariMojtaba Kianmehr
Aug 7, 2019·Analytical and Bioanalytical Chemistry·Giselda BezerraJosé Lima-Filho
Dec 17, 2018·Biosensors & Bioelectronics·Majid SharifiMojtaba Falahati
Feb 24, 2018·Biosensors & Bioelectronics·Yifei WangMeng Lu
Mar 16, 2021·Frontiers in Pharmacology·M ZubairN Ali
Apr 21, 2021·Expert Review of Molecular Diagnostics·Rajesh AhirwarSaroj Kumar
Apr 21, 2019·International Journal of Biological Macromolecules·Ayub KarimzadehNasrin Shadjou
Dec 11, 2019·Talanta·Maria FreitasCristina Delerue-Matos

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