Amperometric phenol biosensor based on laponite clay-chitosan nanocomposite matrix

Biosensors & Bioelectronics
Quan FanSerge Cosnier

Abstract

A novel strategy to fabricate an amperometric biosensor for phenol determination based on chitosan/laponite nanocomposite matrix was described. The composite film was used to immobilize PPO on the surface of a glassy carbon electrode. Chitosan was utilized to improve the analytical performance of the pure clay-modified bioelectrode. The biosensor exhibited a series of properties: good affinity to its substrate (the apparent Michaelis-Menten constant for the sensor was found to be 0.16 mM), high sensitivity (674 mA M(-1)cm(-2) for catechol) and remarkable long-term stability in storage (it retains 88% of the original activity after 60 days). In addition, optimization of the biosensor construction as well as effects of experimental variables such as pH, operating potential and temperature on the amperometric response of the sensor were discussed.

References

Oct 1, 1994·Analytical Biochemistry·R S BrownJ H Luong
Jul 27, 2001·Analytical Chemistry·L Chen, W Gorski
Jan 16, 2002·International Journal of Pharmaceutics·Garnpimol C RitthidejTamotsu Koizumi
Jun 7, 2002·Bioelectrochemistry·Gang WangHong-Yuan Chen
Oct 24, 2003·Analytical Chemistry·Dan ShanChristine Mousty
Aug 17, 2004·Biosensors & Bioelectronics·Dan ShanChristine Mousty

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Citations

Nov 26, 2009·Analytical and Bioanalytical Chemistry·Christine Mousty
Feb 23, 2013·Analytical and Bioanalytical Chemistry·Christine Mousty, Vanessa Prévot
Feb 19, 2009·Applied Biochemistry and Biotechnology·Meral Topcu SulakBülent Keskinler
Sep 30, 2010·Lab on a Chip·S T KoevR Ghodssi
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Lav Kumar SinghMeenakshi Singh
Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sharina Abu HanifahMusa Ahmad
Dec 17, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shou-Nian DingSerge Cosnier
Jan 10, 2013·International Journal of Biological Macromolecules·Thaned PongjanyakulThomas Rades
Apr 20, 2010·International Journal of Pharmaceutics·Wanwisa KhunawattanakulThaned Pongjanyakul
Oct 29, 2015·Journal of Materials Science. Materials in Medicine·Farideh OrdikhaniArash Simchi
Feb 18, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Xuan ZhouYifeng Tu
Apr 25, 2020·Macromolecular Chemistry and Physics·Deepak G Prajapati, Balasubramanian Kandasubramanian
Jul 18, 2019·Scientific Reports·María D AlbaFlorian Raffin
Jul 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·M ElzbieciakP Warszynski
Apr 17, 2021·ACS Biomaterials Science & Engineering·Niranjana Jaya PrakashBalasubramanian Kandasubramanian
Jul 7, 2021·ACS Sensors·Xing XuanGaston A Crespo
Jun 9, 2018·Journal of Agricultural and Food Chemistry·Fuchao ZhanFeng Sheng
Apr 5, 2008·Biosensors & Bioelectronics·Shengfu WangGuodong Liu

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