Amphetamine increases the phosphorylation of neuromodulin and synapsin I in rat striatal synaptosomes

Synapse
S IwataMargaret E Gnegy

Abstract

Amphetamine is taken up through the dopamine transporter in nerve terminals and enhances the release of dopamine. We previously found that incubation of rat striatal synaptosomes increases phosphorylation of the presynaptic neural-specific protein, neuromodulin (Gnegy et al., Mol. Brain Res. 20:289-293, 1993). Using a state-specific antibody, we now demonstrate that incubation of rat striatal synaptosomes with amphetamine increases levels of neuromodulin phosphorylated at ser41, the protein kinase C substrate site. Phosphorylation was maximal at 5 min at 37 degrees C at concentrations from 100 nM to 10 microM amphetamine. The effect of amphetamine on the phosphorylation of synapsin I at a site specifically phosphorylated by Ca2+/calmodulin-dependent protein kinase II (site 3), was examined using a state-specific antibody for site 3-phosphosynapsin I. Incubation with concentrations of amphetamine from 1 to 100 nM increased the level of site 3-phospho-synapsin I at times from 30 sec to 2 min. The effect of amphetamine on synapsin I phosphorylation was blocked by nomifensine. The presence of calcium in the incubating buffer was required for amphetamine to increase the level of site 3-phospho-synapsin I. The amphetamine-mediated in...Continue Reading

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Citations

Aug 26, 1998·Drug and Alcohol Dependence·F J White, P W Kalivas
Feb 7, 1998·Current Opinion in Neurobiology·E J Nestler
Dec 1, 2002·Clinical Neuroscience Research·Todd D GouldHusseini K Manji
Feb 23, 2010·Future Neurology·Rong ChenMargaret E Gnegy
Aug 30, 2011·Neurochemistry International·Omaima M AbdelhafezRasha Z Batran
Nov 11, 2011·CNS Neuroscience & Therapeutics·Fernanda ArmaniJosé Carlos Fernandes Galduróz
Mar 22, 2016·ACS Chemical Neuroscience·Alexander G ZestosMargaret E Gnegy
Feb 7, 2006·Biological Psychiatry·Haim Einat, Husseini K Manji
Oct 15, 2010·The International Journal of Neuropsychopharmacology·Laura MusazziMaurizio Popoli
Sep 3, 2016·Journal of Chemical Neuroanatomy·Sarah MikelmanMargaret E Gnegy
Jul 19, 2005·Physiology·Randy D BlakelyAurelio Galli
Sep 2, 2004·The Journal of Pharmacology and Experimental Therapeutics·L KantorM E Gnegy
Jan 14, 2005·The Journal of Biological Chemistry·L'Aurelle A JohnsonMargaret E Gnegy
Sep 12, 2002·European Journal of Pharmacology·Lana KantorMargaret Gnegy
Nov 13, 2003·European Journal of Pharmacology·Margaret E Gnegy

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