Amphidinolide H, a novel type of actin-stabilizing agent isolated from dinoflagellate

Biochemical and Biophysical Research Communications
Shin-ya SaitoY Ohizumi

Abstract

The effect of novel cytotoxic marine macrolide, amphidinolide H (Amp-H), on actin dynamics was investigated in vitro. Amp-H attenuated actin depolymerization induced by diluting F-actin. This effect remained after washing out of unbound Amp-H by filtration. In the presence of either Amp-H or phalloidin, lag phase, which is the rate-limiting step of actin polymerization, was shortened. Phalloidin decreased the polymerization-rate whereas Amp-H did not. Meanwhile, the effects of both compounds were the same when barbed end of actin was capped by cytochalasin D. Quartz crystal microbalance system revealed interaction of Amp-H with G-actin and F-actin. Amp-H also enhanced the binding of phalloidin to F-actin. We concluded that Amp-H stabilizes actin in a different manner from that of phalloidin and serves as a novel pharmacological tool for analyzing actin-mediated cell function.

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Citations

Jul 10, 2007·Organic Letters·Andreas F PetriCraig M Crews
Jul 26, 2008·The Journal of Antibiotics·Jun'ichi Kobayashi
Feb 3, 2006·Natural Product Reports·John W BluntMichèle R Prinsep
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Apr 19, 2011·Chembiochem : a European Journal of Chemical Biology·Chiara TrigiliIsabel Barasoain
Jun 17, 2015·The Journal of Organic Chemistry·Dani SánchezJaume Vilarrasa
Jul 6, 2019·The Journal of Organic Chemistry·Somnath S Chandankar, Sadagopan Raghavan
Mar 6, 2007·Journal of Natural Products·Jun'ichi Kobayashi, Takaaki Kubota

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