Amphiphilic hollow carbonaceous microsphere-encapsulated enzymes: Facile immobilization and robust biocatalytic properties

Enzyme and Microbial Technology
Li LiuSen Yang

Abstract

This study reports a general strategy for the encapsulation of various enzymes in amphiphilic hollow carbonaceous microspheres (CMs). We found that enzymes could be spontaneously encapsulated in the interior cavity of the CMs via hydrophobic interactions. Due to strong hydrophobic interactions and robust confinement, leaching of the physically adsorbed enzymes is substantially restricted. As a novel immobilization matrix, the CMs display many significant advantages. They are capable of encapsulating a wide range of proteins/enzymes of different sizes, which can then be used in both aqueous and organic media and retain high activity, stability, and excellent reusability. Moreover, CMs could be considered as efficient microreactors that provide a favorable microaqueous environment for enzymes in organic systems. Therefore, this doubly effective and simple immobilization approach can be easily expanded to many other enzymes and has great potential in a variety of enzyme applications.

References

Nov 3, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Kurosch RezwanLudwig J Gauckler
Oct 4, 2008·Angewandte Chemie·Sarah HudsonEdmond Magner
Jan 27, 2009·Chemical Society Reviews·Ulf HanefeldEdmond Magner
Jan 19, 2010·Seikagaku. The Journal of Japanese Biochemical Society·Hiroyasu Ogino
Nov 11, 2011·Advanced Materials·Changzhu WuDayang Wang
Feb 3, 2012·Advanced Materials·Pamela Torres-SalasFrancisco J Plou
Mar 13, 2012·Colloids and Surfaces. B, Biointerfaces·Lei SongBaoshan Xing
Oct 2, 2012·Angewandte Chemie·Zhipeng WangJan C M van Hest
Feb 14, 2013·Chemical Society Reviews·Patrick Adlercreutz
Jun 15, 2013·Chemical Society Reviews·Martin Hartmann, Xenia Kostrov
Sep 17, 2013·Chemical Society Reviews·Lauren BaynePeter J Halling
Oct 12, 2013·Advances in Colloid and Interface Science·Nils CarlssonBjörn Åkerman

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Citations

Aug 2, 2017·Applied Biochemistry and Biotechnology·Sinem Diken GürNilüfer Aksöz
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Jieqing LeiZhigang Luo

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