Amphiphysin IIm is required for survival of Chlamydia pneumoniae in macrophages

The Journal of Experimental Medicine
Elizabeth S GoldAlan Aderem

Abstract

Macrophages play a critical role in both innate and acquired immunity because of their unique ability to internalize, kill, and degrade bacterial pathogens through the process of phagocytosis. The adaptor protein, amphiphysin IIm, participates in phagocytosis and is transiently associated with early phagosomes. Certain pathogens, including Chlamydia pneumoniae, have evolved mechanisms to subvert macrophage phagosome maturation and, thus, are able to survive within these cells. We report here that, although amphiphysin IIm is usually only transiently associated with the phagosome, it is indefinitely retained on vacuoles containing C. pneumoniae. Under these wild-type conditions, C. pneumoniae do not elicit significant nitric oxide (NO) production and are not killed. Abrogation of amphiphysin IIm function results in C. pneumoniae-induced NO production and in the sterilization of the vacuole. The data suggest that C. pneumoniae retains amphiphysin IIm on the vacuole to survive within the macrophage.

References

Sep 1, 1990·The Journal of Infectious Diseases·C C Kuo, J T Grayston
Dec 1, 1986·Journal of Clinical Microbiology·C C KuoJ T Grayston
Sep 1, 1995·Journal of Clinical Microbiology·K L GodzikC C Kuo
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C DavidP de Camilli
Oct 1, 1995·Clinical Microbiology Reviews·C C KuoJ T Grayston
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Aug 28, 1998·Current Opinion in Cell Biology·S L SchmidP De Camilli
Sep 28, 1998·Medical Microbiology and Immunology·B J BalinA P Hudson
Jan 9, 1999·The Journal of Biological Chemistry·D CoxS Greenberg
Mar 20, 1999·The Journal of Biological Chemistry·L E Rameh, L C Cantley
Jun 17, 1999·Rheumatology·T HannuM Leirisalo-Repo
Apr 15, 2000·The Journal of Biological Chemistry·C LeeS G Rhee
Apr 24, 2001·American Journal of Respiratory and Critical Care Medicine·M GencayM Roth
Aug 17, 2002·Science·Eunkyung LeePietro De Camilli
Apr 2, 2003·Journal of Cell Science·Corinne LeprinceJacques Camonis
Jun 13, 2003·Molecular Biology of the Cell·Anke DiH Clive Palfrey
Oct 3, 2003·Cardiovascular Research·Brian B ChesebroLee Ann Campbell
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Dec 9, 2003·The Journal of Biological Chemistry·Philippe SarretAlain Beaudet

❮ Previous
Next ❯

Citations

Jul 23, 2013·Trends in Molecular Medicine·Meng-Shan TanLan Tan
Sep 8, 2011·Infection and Immunity·Jeffrey Mital, Ted Hackstadt

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
Transfections
FCS
density gradient centrifugation
confocal microscopy
FACS
flow cytometry
transmission electron microscopy
Transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.