Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5

Chemical Communications : Chem Comm
Hajra BasitPierre Labbé

Abstract

Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.

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Citations

Oct 23, 2015·Biomacromolecules·Fabrice GiustiManuela Zoonens
Aug 5, 2014·The Journal of Membrane Biology·Yann FerrandezPhilippe Minard
Apr 4, 2014·The Journal of Membrane Biology·Christel Le BonFabrice Giusti
Apr 15, 2014·The Journal of Membrane Biology·Eduardo Antonio Della PiaKaren L Martinez
Jun 28, 2014·The Journal of Membrane Biology·Manuela Zoonens, Jean-Luc Popot
Feb 14, 2015·Protein Expression and Purification·Marjolaine Noirclerc-SavoyeCécile Breyton
Dec 19, 2012·Chemical Communications : Chem Comm·Yi-Chun YehSteven W Singer
May 5, 2018·Frontiers in Molecular Biosciences·Montserrat Serra-BatisteNatàlia Carulla
Jun 9, 2020·Methods : a Companion to Methods in Enzymology·Michaël BoscoGrégory Durand

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