Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics.

Experimental Cell Research
Brian W BoothGilbert H Smith

Abstract

Amphiregulin (AREG), a ligand for epidermal growth factor receptor, is required for mammary gland ductal morphogenesis and mediates estrogen actions in vivo, emerging as an essential growth factor during mammary gland growth and differentiation. The COMMA-D beta-geo (CDbetageo) mouse mammary cell line displays characteristics of normal mammary progenitor cells including the ability to regenerate a mammary gland when transplanted into the cleared fat pad of a juvenile mouse, nuclear label retention, and the capacity to form anchorage-independent mammospheres. We demonstrate that AREG is essential for formation of floating mammospheres by CDbetageo cells and that the mitogen activated protein kinase signaling pathway is involved in AREG-mediated mammosphere formation. Addition of exogenous AREG promotes mammosphere formation in cells where AREG expression is knocked down by siRNA and mammosphere formation by AREG(-/-) mammary epithelial cells. AREG knockdown inhibits mammosphere formation by duct-limited mammary progenitor cells but not lobule-limited mammary progenitor cells. These data demonstrate AREG mediates the function of a subset of mammary progenitor cells in vitro.

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Citations

Feb 17, 2010·Journal of Mammary Gland Biology and Neoplasia·Monique RijnkelsEve Devinoy
Apr 7, 2010·Journal of Mammary Gland Biology and Neoplasia·Katharine M HardyLuigi Strizzi
May 17, 2011·PloS One·Neil R HackettRonald G Crystal
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May 12, 2012·Stem Cells and Development·Jan H SpaasGerlinde R Van de Walle
Dec 4, 2012·Stem Cells and Development·Jang Pyo ParkBrian W Booth
Aug 10, 2011·Future Oncology·Bruno M Simões, Maria Dm Vivanco
Sep 13, 2015·Journal of Mammary Gland Biology and Neoplasia·Corinne A BoulangerGilbert H Smith
Apr 23, 2015·In Vitro Cellular & Developmental Biology. Animal·Jang Pyo ParkBrian W Booth
Jan 1, 2013·Annual Review of Animal Biosciences·Anthony V Capuco, Steven E Ellis
Feb 22, 2017·Journal of Pediatric and Adolescent Gynecology·Frank M BiroSandra Z Haslam
Nov 29, 2017·Cellular Oncology (Dordrecht)·Hannah SchmuckerBrian W Booth
Apr 10, 2013·Journal of Biomaterials Science. Polymer Edition·Brian W BoothKaren J L Burg
Sep 9, 2010·Journal of Mammary Gland Biology and Neoplasia·Erin J McCaveBrian W Booth
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Jul 15, 2021·Developmental Cell·Gat Rauner, Charlotte Kuperwasser

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