Amphotericin B-Loaded Emulgel: Effect of Chemical Enhancers on the Release Profile and Antileishmanial Activity In Vitro

AAPS PharmSciTech
Iluska Martins PinheiroAndré Luis Menezes Carvalho

Abstract

Cutaneous leishmaniasis is a neglected parasitic disease. Treatment is preferably performed with pentavalent antimony associated or not with amphotericin B (AmB). This study aimed to develop an emulgel with different chemical enhancers of cutaneous release. Initially, AmB emulsions were obtained with the chemical promoters, oleic acid and geraniol and without promoter, then for the evaluation of the formulations, a preliminary stability study was carried out where the formulations were submitted to centrifugation, before and after the freeze-thaw cycle and analyzed appearance, color, pH, spreadability, viscosity, conductivity, droplet size, assay, in vitro release study, in vitro antileishmania activity in Leishmania major promastigotes, and macrophage toxicity in the MTT test. The emulsions were yellowish, with no signs of instability after the centrifugation test. The pH range corresponded to that of the skin, which is 4.6 to 5.8, before and after the freeze-thaw cycle, the formulations had good spreadability and did not present significant viscosity differences before and after the freeze-thaw cycle, presenting a non-Newtonian characteristic. AmB content was within the kinetic model of zero order release, the formulation of ...Continue Reading

References

Apr 12, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·P Costa, J M Sousa Lobo
Feb 9, 2008·International Journal of Pharmaceutics·Luana PerioliClaudio Nastruzzi
Nov 15, 2008·Archiv der Pharmazie·Babak Heidary AlizadehAbass Shafiee
Sep 8, 2009·Biochimica Et Biophysica Acta·Pankaj Karande, Samir Mitragotri
Jul 5, 2011·Parasitology International·Diogo G ValadaresEduardo A F Coelho
Jul 9, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society· AjazuddinDulal Krishna Tripathi
Aug 6, 2014·International Journal of Pharmaceutics·Choon Fu Goh, Majella E Lane
Jan 7, 2015·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·V Naga Sravan Kumar VarmaD V Gowda
Feb 24, 2016·Experimental Parasitology·Iluska Martins PinheiroAndré Luis Menezes Carvalho
Jun 24, 2017·Naunyn-Schmiedeberg's Archives of Pharmacology·Michel Muálem de Moraes AlvesFernando Aécio de Amorim Carvalho
Sep 21, 2017·Journal of Immunology Research·Natália Barbosa CarvalhoEdgar Marcelino de Carvalho
Sep 25, 2017·Journal of Pharmaceutical Sciences·Qian ZhangSanjay Garg
Oct 23, 2017·Colloids and Surfaces. B, Biointerfaces·Yu-Hsiang LinPao-Chu Wu
Jul 3, 2018·Drug Development and Industrial Pharmacy·Elvilene de Sousa CoêlhoAndré Luis Menezes Carvalho
Sep 1, 2018·Asian Journal of Pharmaceutical Sciences·Wantanwa KrongrawaChutima Limmatvapirat

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Methods Mentioned

BETA
light microscopy

Software Mentioned

SPSS
GraphPad Prism

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